Shah Md Shahin, Tayab Mohammed Abu, Rahman Anisur, Hasan Muhammad Nazmul, Talukder Md Saddam Hossain, Uddin A M Kafil, Jabed Md, Chy Md Nazim Uddin, Paul Arkajyoti, Rahman Md Masudur, Bin Emran Talha, Seidel Veronique
Department of Pharmacy, International Islamic University Chittagong, Chittagong, 4318, Bangladesh.
Drug Discovery, GUSTO A Research Group, Chittagong, 4203, Bangladesh.
J Tradit Complement Med. 2022 Aug 3;12(6):567-574. doi: 10.1016/j.jtcme.2022.07.001. eCollection 2022 Nov.
This study evaluated the anxiolytic, antidepressant, and antioxidant activity of the methanol extract of (MECR) leaves, and determined the total phenolic and flavonoid contents in this extract.
The anxiolytic effect of MECR (100, 200, 400 mg/kg, p. o.) was tested in mice using the elevated plus-maze (EPM) test, the hole-board test (HBT), and the light-dark box (LDB) test. Its antidepressant effect was evaluated in the tail suspension (TST) and the forced swim (FST) tests. The total phenolic (TPC) and flavonoid (TFC) content was measured using standard colorimetric assays. Antioxidant activity was determined using the DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays.
MECR, at all doses, showed dose-dependent anxiolytic activity. At 400 mg/kg, it significantly increased the time spent and number of entries in the open arms (EPM test), the number of head-dips (HBT), and the time spent into the light compartment (LDB) test compared to the control. In the TST and FST, MECR dose-dependently reduced the duration of immobility compared to untreated animals. This was significant for all doses except for 100 mg/kg in the FST model. MECR showed high TPC and TFC (90.94 ± 0.75 mg GAE/g and 51.54 ± 0.78 mg QE/g of dried extract, respectively) and displayed potent activity in the DPPH radical scavenging (IC = 177.82 μg/mL) and FRAP assays. These findings indicate that has the potential to alleviate anxiety and depression disorders, which merits further exploration.
本研究评估了[植物名称]（MECR）叶甲醇提取物的抗焦虑、抗抑郁和抗氧化活性，并测定了该提取物中的总酚和黄酮含量。
采用高架十字迷宫（EPM）试验、洞板试验（HBT）和明暗箱试验（LDB），在小鼠中测试MECR（100、200、400mg/kg，口服）的抗焦虑作用。通过悬尾试验（TST）和强迫游泳试验（FST）评估其抗抑郁作用。使用标准比色法测定总酚（TPC）和黄酮（TFC）含量。通过DPPH自由基清除和铁还原抗氧化能力（FRAP）试验测定抗氧化活性。
所有剂量的MECR均表现出剂量依赖性抗焦虑活性。与对照组相比，在400mg/kg时，它显著增加了在开放臂的停留时间和进入次数（EPM试验）、头部浸入次数（HBT）以及在明室的停留时间（LDB试验）。在TST和FST中，与未处理动物相比，MECR剂量依赖性地减少了不动时间。除FST模型中100mg/kg剂量外，所有剂量均有显著差异。MECR显示出高TPC和TFC（分别为90.94±0.75mg GAE/g和51.54±0.78mg QE/g干提取物），并在DPPH自由基清除试验（IC=177.82μg/mL）和FRAP试验中表现出强活性。这些发现表明[植物名称]有减轻焦虑和抑郁障碍的潜力，值得进一步探索。
