Mejia-Trujillo Raquel, Smith Chase H
Department of Integrative Biology, University of Texas, Austin, TX, USA.
Mitochondrial DNA B Resour. 2022 Oct 27;7(10):1854-1858. doi: 10.1080/23802359.2022.2134750. eCollection 2022.
The global decline of freshwater mussels emphasizes the need to establish genetic resources to better understand their biology, including a unique mitochondrial biology known as doubly uniparental inheritance. In this study, we present the complete male-type (M-type) mitochondrial genome of the freshwater mussel, Smith, Johnson, Inoue, Doyle, & Randklev, 2019. The M-type mtDNA is approximately 16 kilobases and contains 22 tRNAs, two rRNAs, and 14 protein-coding genes, including a male-specific open reading frame. Read coverage revealed that M-type mtDNA was more abundant than female-type mtDNA in male gonadal tissue, with respect to a non-spawning male individual. Novel mitogenomes were resolved within previously described sex-specific monophyletic clades across the subfamily Ambleminae. The availability of high-quality nuclear and mitochondrial genomic data for makes it a model for future research into the potential role of mtDNA in sex determination or sexual development in freshwater mussels.
全球淡水贻贝数量的减少凸显了建立遗传资源以更好地了解其生物学特性的必要性，包括一种独特的线粒体生物学现象，即双单亲遗传。在本研究中，我们展示了淡水贻贝的完整雄性型（M型）线粒体基因组（史密斯、约翰逊、井上、多伊尔和兰德克莱夫，2019年）。M型线粒体DNA约为16千碱基，包含22个tRNA、两个rRNA和14个蛋白质编码基因，包括一个雄性特异性开放阅读框。读取覆盖率显示，对于一个未产卵的雄性个体，在雄性性腺组织中M型线粒体DNA比雌性型线粒体DNA更为丰富。在先前描述的 across亚科的性别特异性单系分支中解析出了新的有丝分裂基因组。 高质量的核基因组和线粒体基因组数据的可用性使其成为未来研究线粒体DNA在淡水贻贝性别决定或性发育中潜在作用的模型。
