维生素 D 合成酶 CYP27B1 在系统性红斑狼疮中的作用。
The role of vitamin D-synthesizing enzyme CYP27B1 in systemic lupus erythematosus.
机构信息
Division of Rheumatology, Department of Internal Medicine, Suining Central Hospital, Sichuan, China ; Division of Institute Rheumatology, Department of Internal Medicine, Faculty of Medicine, Affiliated Hospital of North Sichuan Medical College, Sichuan, China.
Division of Institute Rheumatology, Department of Internal Medicine, Faculty of Medicine, Affiliated Hospital of North Sichuan Medical College, Sichuan, China.
出版信息
Turk J Med Sci. 2022 Aug;52(4):984-989. doi: 10.55730/1300-0144.53899. Epub 2022 Aug 10.
BACKGROUND
To measure the expression of 1α-hydroxylase (CYP27B1) and serum 25(OH)D concentration in systemic lupus erythematosus (SLE) and to investigate the role of CYP27B1 in SLE.
METHODS
Seventy-seven SLE patients and 35 healthy controls (HCs) were enrolled from September 2017 to January 2020. The study design is cross-sectional. mRNA expression of CYP27B1 in peripheral blood mononuclear cells (PBMCs) was measured by reverse-transcription quantitative PCR, the protein level of CYP27B1 was quantified by western blotting, and the serum level of 25(OH) D was determined by an enzyme-linked immunosorbent assay.
RESULTS
The mRNA expression of CYP27B1 in PBMCs was significantly lower in SLE patients than in HCs (p < 0.001), and the protein quantification confirmed that CYP27B1 expression was lower in SLE patients than in HCs (p = 0.001). Among SLE patients, the prevalence of lupus nephritis was higher in a subgroup with lower CYP27B1 mRNA expression than in a subgroup with normal CYP27B1 mRNA expression (41.07% vs. 14.28%, p = 0.028). The mRNA expression of CYP27B1 negatively correlated with the Systemic Lupus Erythematosus Disease Activity Index (r = -0.331, p = 0.003). Serum 25(OH)D concentration was lower in SLE patients than in HCs (37.64 ± 19.89 vs. 50.58 ± 12.74 ng/mL, mean ± SD, p = 0.003).
DISCUSSION
The expression of CYP27B1 in PBMCs may be related to SLE pathogenesis, disease activity, and nephritis.
背景
检测系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中 1α-羟化酶（CYP27B1）的表达及血清 25（OH）D 浓度，探讨 CYP27B1 在 SLE 发病中的作用。
方法
本研究采用病例对照研究设计，收集 2017 年 9 月至 2020 年 1 月期间的 77 例 SLE 患者和 35 例健康对照者的外周血标本，应用实时荧光定量 PCR 检测 CYP27B1 的 mRNA 表达，Western blot 检测 CYP27B1 蛋白表达，酶联免疫吸附法检测血清 25（OH）D 浓度。
结果
与健康对照组相比，SLE 患者外周血单个核细胞中 CYP27B1 的 mRNA 表达明显降低（P<0.001），且 CYP27B1 蛋白表达也显著降低（P=0.001）。在 SLE 患者中，CYP27B1mRNA 低表达组狼疮肾炎的发生率明显高于 CYP27B1mRNA 正常表达组（41.07%比 14.28%，P=0.028）。CYP27B1mRNA 表达与系统性红斑狼疮疾病活动指数（SLEDAI）评分呈负相关（r=-0.331，P=0.003）。SLE 患者的血清 25（OH）D 浓度明显低于健康对照组（37.64±19.89比 50.58±12.74ng/ml，P=0.003）。
讨论
PBMC 中 CYP27B1 的表达可能与 SLE 的发病机制、疾病活动度及狼疮肾炎有关。
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