Mishima Marcella Duarte Villas, da Silva Bárbara Pereira, Gomes Mariana Juste Contin, Toledo Renata Celi Lopes, Pereira Carlos Eduardo Real, Costa Neuza Maria Brunoro, Martino Hércia Stampini Duarte
Department of Nutrition and Health, Federal University of Viçosa/UFV, Viçosa, MG, 36570-900, Brazil.
Department of Veterinary, Federal University of Viçosa/UFV, Viçosa, MG, 36570-900, Brazil.
Eur J Nutr. 2023 Mar;62(2):905-919. doi: 10.1007/s00394-022-03043-2. Epub 2022 Nov 3.
The present study aimed to evaluate the effect of chia flour associated with a high fat diet on intestinal health in female ovariectomized Wistar rats.
The study was conducted with 32 adult female ovariectomized Wistar rats, which were separated into four groups: standard diet (ST), standard diet with chia (STC), high fat diet (HF) and high fat diet with chia (HFC) for 18 weeks. Cecum content pH, short chain fatty acid content, brush border membrane functionality and morphology and the gut microbiota were evaluated.
This study demonstrated that the consumption of chia flour increased the production of acetic and butyric acids, the longitudinal and circular muscle layers and crypt thickness. It also improved the expression of aminopeptidase (AP) and sucrose-isomaltase (SI) and decreased the cecum content pH. Further, the consumption of chia improved richness and decreased diversity of the microbiota. Operational Taxonomic Units (OTUs) clustering indicated difference between the ST and STC groups. In the linear discriminant analysis effect size (LEfSe) analysis, the Bacteroides genus and members of the Muribaculaceae and Lachnospiraceae families were enriched in the STC treatment group. The STC group demonstrated the enrichment of Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways related to peptidoglycan and coenzyme A biosynthesis.
Our results suggest that chia flour, which is rich in dietary fiber and phenolic compounds, presented potential properties to improve intestinal health.
本研究旨在评估奇亚籽粉与高脂饮食联合对雌性去卵巢Wistar大鼠肠道健康的影响。
对32只成年雌性去卵巢Wistar大鼠进行研究，将其分为四组：标准饮食组（ST）、含奇亚籽的标准饮食组（STC）、高脂饮食组（HF）和含奇亚籽的高脂饮食组（HFC），持续18周。评估盲肠内容物pH值、短链脂肪酸含量、刷状缘膜功能和形态以及肠道微生物群。
本研究表明，食用奇亚籽粉可增加乙酸和丁酸的产生、纵肌层和环肌层以及隐窝厚度。它还改善了氨肽酶（AP）和蔗糖异麦芽糖酶（SI）的表达，并降低了盲肠内容物pH值。此外，食用奇亚籽可改善微生物群的丰富度并降低其多样性。操作分类单元（OTU）聚类表明ST组和STC组之间存在差异。在线性判别分析效应大小（LEfSe）分析中，拟杆菌属以及毛螺菌科和瘤胃菌科的成员在STC处理组中富集。STC组显示出与肽聚糖和辅酶A生物合成相关的京都基因与基因组百科全书（KEGG）代谢途径的富集。
我们的结果表明，富含膳食纤维和酚类化合物的奇亚籽粉具有改善肠道健康的潜在特性。
