Department of Nutrition and Health, Federal University of Viçosa, Av. Purdue, s/n, Campus Universitário, Viçosa, MG 36.570-900, Brazil.
Plant Biotechnology Program, Vila Velha University, Av. Comissário José Dantas de Melo, n 21, Vila Velha, ES 29102-623, Brazil.
Food Funct. 2019 Jul 17;10(7):4036-4045. doi: 10.1039/c9fo00862d.
The present study investigated the influence of chia consumption on inflammation, oxidative stress, and lipid profiles in adult female ovariectomized rats fed a high-fat diet. Forty ovariectomized and 40 intact (SHAM) rats were allocated into 8 groups (n = 10), and each rat received one of the following four diets: standard diet (ST); standard diet + chia (STC); high-fat diet (HF); and high-fat diet + chia (HFC) for 126 days. Biochemical parameters and biomarkers of lipid peroxidation, inflammation, and oxidative stress were evaluated. The mRNA expression levels of PPAR-α, NFκB, TNF-α and Zn-SOD1 were analyzed, as well as those of TNF-α and IL-1β. Chia intake increased HDL cholesterol (HDL-c) and reduced LDL cholesterol (LDL-c) levels. Plasma catalase activity was elevated in the STC group. Concentrations of TBARS were higher in all groups fed HF. PPAR-α mRNA expression was elevated, and levels of NFκB mRNA expression were reduced in the STC group. mRNA expression and protein levels of TNF-α were lower in rats fed the standard diet. Protein levels of IL-1β were reduced in rats fed the standard diet, and the high fat diet with chia. In general, ovariectomy did not influence the inflammatory and oxidative stress parameters. Chia intake improved antioxidant activity by increasing SOD expression, PPAR-α expression, catalase activity, and HDL-c levels. In addition, chia consumption decreased the concentrations of the inflammatory markers IL-1β and LDL-c.
本研究旨在探讨奇亚籽对高脂饮食喂养的去卵巢成年雌性大鼠的炎症、氧化应激和血脂谱的影响。将 40 只去卵巢和 40 只假手术（SHAM）大鼠分为 8 组（每组 10 只），每只大鼠接受以下四种饮食中的一种：标准饮食（ST）；标准饮食+奇亚籽（STC）；高脂饮食（HF）；高脂饮食+奇亚籽（HFC），共 126 天。评估了生化参数和脂质过氧化、炎症和氧化应激的生物标志物。分析了 PPAR-α、NFκB、TNF-α和 Zn-SOD1 的 mRNA 表达水平，以及 TNF-α和 IL-1β的 mRNA 表达水平。奇亚籽的摄入增加了高密度脂蛋白胆固醇（HDL-c），降低了低密度脂蛋白胆固醇（LDL-c）水平。STC 组血浆过氧化氢酶活性升高。所有 HF 喂养组的 TBARS 浓度升高。PPAR-α mRNA 表达上调，STC 组 NFκB mRNA 表达下调。标准饮食喂养的大鼠 TNF-α mRNA 表达和蛋白水平降低。标准饮食和高脂肪饮食加奇亚籽喂养的大鼠 IL-1β蛋白水平降低。一般来说，去卵巢对炎症和氧化应激参数没有影响。奇亚籽的摄入通过增加 SOD 表达、PPAR-α表达、过氧化氢酶活性和 HDL-c 水平来改善抗氧化活性。此外，奇亚籽的摄入降低了炎症标志物 IL-1β和 LDL-c 的浓度。
