Am J Vet Res. 2022 Nov 7;83(12):ajvr.22.07.0120. doi: 10.2460/ajvr.22.07.0120.
To determine luteinizing hormone receptor (LHR) expression and response to LHR activation in isolated canine splenic hemangiosarcoma cell lines in vitro.
In vitro cultures of commercially available canine splenic hemangiosarcoma cell lines (EFS, GRACE-HSA, and DAL-4).
The percentage of each cell line expressing LHR was determined by immunocytochemistry. Cells were then treated with increasing doses (7.5 ng/mL, 75 ng/mL) of recombinant canine luteinizing hormone (cLH) for 48 hours and evaluated using a cell proliferation assay.
The percentage of cells expressing LHR was 17.2 ± 4.5%, 11.8 ± 3.1%, and 6.9 ± 2.5% in EFS, GRACE-HSA, and DAL-4, respectively. There was significant increase in cell count in the DAL-4 and EFS cell lines following a 48-hour incubation at the highest cLH concentration (P = .028 and P = .019, respectively). There was not a significant increase in cell count in the GRACE-HSA cell line at either cLH concentration.
Activation of LHR results in cell proliferation in some canine splenic hemangiosarcoma cell lines. These results may explain why spayed and castrated dogs with high circulating LH concentrations may develop hemangiosarcoma more frequently than intact dogs.
确定犬脾血管肉瘤细胞系中黄体生成素受体 (LHR) 的表达及其对 LHR 激活的反应。
市售犬脾血管肉瘤细胞系 (EFS、GRACE-HSA 和 DAL-4) 的体外培养物。
通过免疫细胞化学测定每种细胞系中 LHR 表达的百分比。然后用不同剂量（7.5ng/mL、75ng/mL）的重组犬促黄体激素（cLH）处理细胞 48 小时，并通过细胞增殖测定进行评估。
EFS、GRACE-HSA 和 DAL-4 细胞系中 LHR 表达的细胞百分比分别为 17.2±4.5%、11.8±3.1%和 6.9±2.5%。在最高 cLH 浓度孵育 48 小时后，DAL-4 和 EFS 细胞系中的细胞计数显著增加（P=0.028 和 P=0.019）。在两种 cLH 浓度下，GRACE-HSA 细胞系中的细胞计数均无显著增加。
LHR 的激活导致一些犬脾血管肉瘤细胞系的细胞增殖。这些结果可能解释了为什么循环中 LH 浓度较高的去势和绝育犬比未绝育犬更频繁地发生血管肉瘤。
