Marques Ana M, Petrucci Gonçalo, Gregório Hugo, Lobo Luís, Henriques Joaquim, Figueira Ana C, Vilhena Hugo, Marrinhas Carla, Queiroga Felisbina L
Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.
Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Escola Universitária Vasco da Gama (EUVG), Av. José R Sousa Fernandes, 297, 3020-210 Coimbra, Portugal.
Vet Sci. 2025 Apr 9;12(4):346. doi: 10.3390/vetsci12040346.
Peripheral complete blood cell count (CBC) and blood ratios, including neutrophil-to-lymphocyte ratio (NLR), neutrophil-to-red blood cell ratio (NRR), and platelet-to-lymphocyte ratio (PLR), have been used in the diagnosis and prognosis of several cancers; however, their relevance in canine splenic hemangiosarcoma (HSA) remains to be investigated. This study investigated whether CBC, NLR, NRR, and PLR could be diagnostic and prognostic biomarkers in dogs with splenic HSA. Analyzing medical records of 154 dogs undergoing splenectomy from 2018 to 2022, we found that dogs diagnosed with splenic HSA (n = 63) had significantly higher neutrophil counts (14.9 ± 9.7 vs. 12.6 ± 9.6; < 0.001), increased NRR (3.7 ± 2.6 vs. 2.7 ± 3.7; < 0.001), lower platelet counts (145 ± 111 vs. 270 ± 213; < 0.001), and reduced PLR (139.4 ± 160.0 vs. 259.9 ± 278.0; < 0.001) compared to dogs with other splenic lesions. This study also identified a higher risk of relapse and mortality associated with increased NRR ( < 0.001 and = 0.012, respectively) and an inverse relationship with PLR ( = 0.015 and = 0.033, respectively), whereas NLR showed no significant association. The multivariate survival analysis identified NRR as an independent prognostic factor for DFI [hazard ratio (1.837); 95% confidence interval (1.147-2.942); = 0.011], while for OS, the association did not reach statistical significance [hazard ratio (1.510); 95% confidence interval (0.985-2.314); = 0.059]. These findings highlight the potential of NRR and PLR as biomarkers for assessing diagnosis and prognosis in canine splenic HSA, advocating for further validation in the future.
外周全血细胞计数（CBC）和血液比率，包括中性粒细胞与淋巴细胞比率（NLR）、中性粒细胞与红细胞比率（NRR）以及血小板与淋巴细胞比率（PLR），已被用于多种癌症的诊断和预后评估；然而，它们在犬脾脏血管肉瘤（HSA）中的相关性仍有待研究。本研究调查了CBC、NLR、NRR和PLR是否可作为犬脾脏HSA的诊断和预后生物标志物。通过分析2018年至2022年接受脾切除术的154只犬的病历，我们发现，与患有其他脾脏病变的犬相比，被诊断为脾脏HSA的犬（n = 63）中性粒细胞计数显著更高（14.9±9.7对12.6±9.6；<0.001），NRR升高（3.7±2.6对2.7±3.7；<0.001），血小板计数更低（145±111对270±213；<0.001），PLR降低（139.4±160.0对259.9±278.0；<0.001）。本研究还发现，NRR升高与复发和死亡风险增加相关（分别为<0.001和 = 0.012），与PLR呈负相关（分别为 = 0.015和 = 0.033），而NLR未显示出显著关联。多因素生存分析确定NRR是无病生存期（DFI）的独立预后因素[风险比（1.837）；95%置信区间（1.147 - 2.942）； = 0.011]，而对于总生存期（OS），该关联未达到统计学显著性[风险比（1.510）；95%置信区间（0.985 - 2.314）； = 0.059]。这些发现突出了NRR和PLR作为评估犬脾脏HSA诊断和预后生物标志物的潜力，提倡未来进行进一步验证。
