竹叶提取物及其成分香豆酸甲酯的细胞抗炎和抗氧化活性。

Cellular Anti-Inflammatory and Antioxidant Activities of Bamboo Leaf Extract and Its Constituent Coumaric Acid Methyl Ester.

机构信息

Fukuyu Medical Institute, Fukuyu Medical Corporation, Nisshin, Aichi 470-0103, Japan.

Department of Molecular Target Medicine, Aichi Medical University, Nagakute 480-1195, Japan.

出版信息

ScientificWorldJournal. 2022 Oct 25;2022:8454865. doi: 10.1155/2022/8454865. eCollection 2022.

DOI:10.1155/2022/8454865

PMID:36330350

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9626218/

Abstract

BACKGROUND

Hot water extract of (Kumazasa) leaves is commercially available and used as a dietary supplement or skincare cream. The extract possesses anti-inflammatory activity on the mouse atopic dermatitis model. To elucidate the mechanism of in vivo activity, we have studied the cellular anti-inflammatory and antioxidant activities of the extract and its constituents.

METHODS

Secretion of mouse and human IL-6 was measured by ELISA. ROS production was measured by a fluorescent reagent. Ultrahigh performance liquid chromatography (UHPLC)/MS was used for the ingredient analysis.

RESULTS

The extract inhibited inflammatory mediators such as LPS-induced NO, IL-6, and ROS production in mouse monocyte leukemia RAW264.7 cells. It also inhibited iNOS, IL-6, and IL-1 expressions. Moreover, it inhibited LPS-induced IL-6 expression and production in human monocyte leukemia THP-1 cells differentiated into macrophages. The HPLC analysis of the extract revealed the existence of coumaric acid, ferulic acid, and coumaric acid methyl ester. Coumaric acid methyl ester but not coumaric acid or ferulic acid inhibited LPS-induced NO, IL-6, and ROS production in RAW264.7 cells and IL-6 production in differentiated THP-1 cells.

CONCLUSION

The hot water extract of leaves and one of its constituents possess cellular anti-inflammatory and antioxidant activities.

摘要

背景

（桑白皮）叶子的热水提取物可在市面上买到，并被用作膳食补充剂或护肤霜。该提取物对小鼠特应性皮炎模型具有抗炎活性。为了阐明体内活性的机制，我们研究了提取物及其成分的细胞抗炎和抗氧化活性。

方法

通过 ELISA 测量小鼠和人 IL-6 的分泌。通过荧光试剂测量 ROS 的产生。使用超高效液相色谱（UHPLC）/MS 进行成分分析。

结果

该提取物抑制了小鼠单核细胞白血病 RAW264.7 细胞中 LPS 诱导的 NO、IL-6 和 ROS 产生等炎症介质。它还抑制了 iNOS、IL-6 和 IL-1 的表达。此外，它还抑制了人单核细胞白血病 THP-1 细胞分化为巨噬细胞后 LPS 诱导的 IL-6 表达和产生。提取物的 HPLC 分析表明存在香豆酸、阿魏酸和香豆酸甲酯。香豆酸甲酯而非香豆酸或阿魏酸抑制了 RAW264.7 细胞中的 LPS 诱导的 NO、IL-6 和 ROS 产生以及分化的 THP-1 细胞中的 IL-6 产生。