Sakagami Hiroshi, Nakatani Sachie, Enomoto Ayame, Ota Sana, Kaneko Miku, Sugimoto Masahiro, Horiuchi Misaki, Toeda Kazuki, Oizumi Takaaki
Meikai University Research Institute of Odontology (M-RIO), Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan.
Graduate School of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
J Clin Med. 2021 May 13;10(10):2100. doi: 10.3390/jcm10102100.
Efficient utilization of alkaline extracts of several plants for the treatment of oral diseases has been reported. To investigate the mechanism of anti-inflammatory activity of alkaline extract of the leaves of sp. (SE), multi-omics analysis using metabolomics and DNA array was performed. Human gingival fibroblasts (HGFs) were treated for IL-1β to induce inflammation (detected by PGE production in culture medium) in the presence or absence of SE. Both IL-1β and SE showed slight hormetic growth stimulation against HGF. SE inhibited PGE production dose- and time-dependently. Its inhibitory action was more pronounced by first treating the cells with SE, rather than with IL-1β. At 3 h after IL-1β treatment, 18 amino acids (except cysteine and glutamic acid), total glutathione (GSH, GSSG, Cys-GSH disulfide), Met-sulfoxide, 5-oxoproline, and SAM declined, whereas DNA expressions of AKT, CASP3, and CXCL3 were elevated. These changes were reversed by simultaneous treatment with SE. The present study suggests that the anti-inflammatory action of SE is mediated via various metabolic pathways for cell survival, apoptosis, and leukocyte recruitment.
已有报道称几种植物的碱性提取物可有效用于治疗口腔疾病。为了研究某植物叶片碱性提取物（SE）的抗炎活性机制，进行了代谢组学和DNA阵列的多组学分析。在有或无SE存在的情况下，用人牙龈成纤维细胞（HGF）处理白细胞介素-1β（IL-1β）以诱导炎症（通过培养基中前列腺素E的产生来检测）。IL-1β和SE对HGF均表现出轻微的刺激生长效应。SE剂量和时间依赖性地抑制前列腺素E的产生。通过先用SE而非IL-1β处理细胞，其抑制作用更为明显。在IL-1β处理3小时后，18种氨基酸（除半胱氨酸和谷氨酸外）、总谷胱甘肽（GSH、GSSG、半胱氨酸-谷胱甘肽二硫化物）、甲硫氨酸亚砜、5-氧代脯氨酸和S-腺苷甲硫氨酸下降，而AKT、CASP3和CXCL3的DNA表达升高。同时用SE处理可逆转这些变化。本研究表明，SE的抗炎作用是通过细胞存活、凋亡和白细胞募集的各种代谢途径介导的。
