Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 34054, Republic of Korea.
Research Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44429, Republic of Korea.
J Microbiol Biotechnol. 2022 Dec 28;32(12):1615-1621. doi: 10.4014/jmb.2209.09031. Epub 2022 Oct 28.
Tissue regeneration is the ultimate treatment for many degenerative diseases, however, repair and regeneration of damaged organs or tissues remains a challenge. Previously, we showed that B1 Ab and H3 Ab induce stem cells to differentiate into microglia and brown adipocyte-like cells, while trafficking to the brain and heart, respectively. Here, we present data showing that another selected agonist antibody, P1 antibody, induces the migration of cells to the pancreatic islets and differentiates human stem cells into beta-like cells. Interestingly, our results suggest the purified P1 Ab induces beta-like cells from fresh, human CD34 hematopoietic stem cells and mouse bone marrow. In addition, stem cells with P1 Ab bound to expressed periostin (POSTN), an extracellular matrix protein that regulates tissue remodeling, selectively migrate to mouse pancreatic islets. Thus, these results confirm that our in vivo selection system can be used to identify antibodies from our library which are capable of inducing stem cell differentiation and cell migration to select tissues for the purpose of regenerating and remodeling damaged organ systems.
组织再生是许多退行性疾病的终极治疗方法，然而，受损器官或组织的修复和再生仍然是一个挑战。以前，我们已经证明 B1 抗体和 H3 抗体分别诱导干细胞分化为小胶质细胞和棕色脂肪细胞样细胞，并向大脑和心脏迁移。在这里，我们提供的数据表明，另一种选择的激动剂抗体 P1 抗体诱导细胞向胰岛迁移，并将人干细胞分化为类似β细胞的细胞。有趣的是，我们的结果表明，从新鲜的人 CD34 造血干细胞和小鼠骨髓中纯化的 P1 抗体可以诱导类似β细胞。此外，与细胞外基质蛋白骨桥蛋白（POSTN）结合的 P1 抗体表达的干细胞选择性地迁移到小鼠胰岛。因此，这些结果证实，我们的体内选择系统可用于鉴定我们文库中的抗体，这些抗体能够诱导干细胞分化和细胞迁移，以选择组织来再生和重塑受损的器官系统。
