Rettig W J, Real F X, Spengler B A, Biedler J L, Old L J
Science. 1986 Mar 14;231(4743):1281-4. doi: 10.1126/science.3633135.
Human malignant melanoma cells express specific chondroitin sulfate proteoglycans (mel-CSPG) on the surface, both in vivo and in vitro. Melanocytes in normal skin show no detectable mel-CSPG but can be induced to express the antigen when cultured in the presence of cholera toxin and the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. Most other cell types do not express mel-CSPG either in vivo or in vitro. A study was designed to examine regulatory signals controlling mel-CSPG expression. The gene encoding mel-CSPG was mapped to human chromosome 15, and this chromosome was introduced into rodent cells derived from distinct differentiation lineages. Three types of mel-CSPG--expressing hybrids were found: (i) hybrids derived from human melanomas; (ii) hybrids derived from human cells that do not express mel-CSPG; and (iii) hybrids derived from human cells expressing mel-CSPG that are antigen-negative but that are induced to express mel-CSPG when cultured on extracellular matrix instead of plastic surfaces. Thus, mel-CSPG expression can be controlled both through intrinsic signals, provided by the differentiation program of the rodent fusion partner, and through extrinsic signals, provided by specific cell-matrix interactions.
人类恶性黑色素瘤细胞在体内和体外均在表面表达特定的硫酸软骨素蛋白聚糖（mel-CSPG）。正常皮肤中的黑素细胞未检测到mel-CSPG，但在霍乱毒素和肿瘤启动子十四烷酰佛波醇乙酯存在的情况下培养时可被诱导表达该抗原。大多数其他细胞类型在体内或体外均不表达mel-CSPG。设计了一项研究来检查控制mel-CSPG表达的调节信号。编码mel-CSPG的基因被定位到人类15号染色体上，并将该染色体导入源自不同分化谱系的啮齿动物细胞中。发现了三种表达mel-CSPG的杂种细胞：（i）源自人类黑色素瘤的杂种细胞；（ii）源自不表达mel-CSPG的人类细胞的杂种细胞；（iii）源自表达mel-CSPG但抗原阴性的人类细胞的杂种细胞，这些细胞在细胞外基质而非塑料表面上培养时被诱导表达mel-CSPG。因此，mel-CSPG的表达既可以通过啮齿动物融合伙伴的分化程序提供的内在信号来控制，也可以通过特定细胞-基质相互作用提供的外在信号来控制。
