Uddin Mohammed Jafar, Haque Farhana, Jabeen Ishrat, Shuvo Sabbir R
Department of Biochemistry & Microbiology, School of Health & Life Sciences, North South University, Dhaka, Bangladesh.
Can J Microbiol. 2023 Jan 1;69(1):44-52. doi: 10.1139/cjm-2022-0149. Epub 2022 Nov 4.
SRS1, gram-negative bacteria, were isolated from Savar, Bangladesh. The strain could tolerate up to 80 mmol L sodium arsenite, 400 mmol L sodium arsenate, 5 mmol L manganese sulfate, 3 mmol L lead nitrate, 2.5 mmol L cobalt chloride, 2.5 mmol L cadmium acetate, and 2.5 mmol L chromium chloride. The whole-genome sequencing revealed that the genome size of SRS1 is estimated to be 5.4 Mb long, and the G + C content is 51.7%. The genome of SRS1 contains , , , , , , and genes for arsenic resistance; , , , and genes for cobalt resistance; and genes for chromium resistance; , , , , and genes for manganese resistance; and gene for lead and cadmium resistance. This novel gene has never previously been reported in any strain except SRS1. A set of 130 completely sequenced strains of was selected for phylogenomic analysis. The phylogenetic tree showed that the SRS1 strain is closely related to the 62 strain. Further analyses of the genes involved in metal and metalloid resistance might facilitate identifying the mechanisms and pathways involved in high metal resistance in the SRS1 strain.
SRS1，一种革兰氏阴性菌，从孟加拉国萨瓦尔分离得到。该菌株能够耐受高达80毫摩尔/升的亚砷酸钠、400毫摩尔/升的砷酸钠、5毫摩尔/升的硫酸锰、3毫摩尔/升的硝酸铅、2.5毫摩尔/升的氯化钴、2.5毫摩尔/升的醋酸镉和2.5毫摩尔/升的氯化铬。全基因组测序显示，SRS1的基因组大小估计为5.4兆碱基对长，G + C含量为51.7%。SRS1的基因组包含用于抗砷的arsA、arsB、arsC、arsD、arsE、arsF和arsG基因；用于抗钴的cobA、cobN、cobM和cobO基因；用于抗铬的chrA和chrB基因；用于抗锰的mntA、mntB、mntC、mntD和mntE基因；以及用于抗铅和镉的czcA基因。这种新的czcA基因此前从未在除SRS1之外的任何菌株中报道过。选择了一组130个已完成全序列测定的菌株进行系统基因组分析。系统发育树显示，SRS1菌株与62菌株密切相关。对参与金属和类金属抗性的基因进行进一步分析，可能有助于确定SRS1菌株中高金属抗性所涉及的机制和途径。
