Haque Farhana, Jabeen Ishrat, Keya Chaman Ara, Shuvo Sabbir R
Department of Biochemistry & Microbiology, School of Health & Life Sciences, North South University, Dhaka, Bangladesh.
AIMS Microbiol. 2022 Jun 24;8(2):227-239. doi: 10.3934/microbiol.2022018. eCollection 2022.
Heavy metal contamination of the environment is a primary concern in Bangladesh. This study aims to characterize a novel heavy metal tolerant strain, FHq, isolated from the tannery effluents of Savar, Bangladesh. The strain could tolerate up to 5 mM of lead nitrate, 2.5 mM of sodium arsenate, chromium chloride, cobalt chloride, 1.5 mM cadmium acetate, and 1 mM of sodium arsenite. Whole-genome sequencing analysis revealed that the genome of the strain is around 5.2 Mbp long, and the G + C content is 35.4%. Besides, FHq has genes c, and c, which confer lead, arsenic, cobalt, and chromium resistance, respectively. A total of nineteen other closely related and completely sequenced strains were selected based on average nucleotide identity along with the FHq strain for phylogenomic and pan-genome analysis. The phylogenomic analysis predicted the inter-genomic evolutionary relationship of the strain isolated from Bangladesh, and it was closely related to a strain isolated from China. Pan-genome analysis revealed that the FHq strain possesses 6045 pan genes, 3802 core genes, and 152 unique genes in its genomic content. Hence, the genetic information and comparative analysis of the FHq strain might facilitate identifying the mechanisms conferring high resistance to lead in strains isolated from Bangladesh.
环境中的重金属污染是孟加拉国的一个主要问题。本研究旨在鉴定一种从孟加拉国萨瓦尔制革厂废水中分离出的新型重金属耐受菌株FHq。该菌株能够耐受高达5 mM的硝酸铅、2.5 mM的砷酸钠、氯化铬、氯化钴、1.5 mM的醋酸镉和1 mM的亚砷酸钠。全基因组测序分析表明，该菌株的基因组长度约为5.2 Mbp，G + C含量为35.4%。此外，FHq具有分别赋予铅、砷、钴和铬抗性的基因c和c。基于平均核苷酸同一性，共选择了另外19个密切相关且已完成测序的菌株，与FHq菌株一起进行系统基因组学和泛基因组分析。系统基因组学分析预测了从孟加拉国分离出的该菌株的基因组间进化关系，它与从中国分离出的一个菌株密切相关。泛基因组分析表明，FHq菌株在其基因组内容中拥有6045个泛基因、3802个核心基因和152个独特基因。因此，FHq菌株的遗传信息和比较分析可能有助于确定从孟加拉国分离出的菌株中赋予对铅高抗性的机制。
