Vogt W, Damerau B, Lühmann B, Hesse D, Haller Y
Int Arch Allergy Appl Immunol. 1986;79(4):423-33. doi: 10.1159/000234013.
Complement components, their activation and the generation of C3a and C5a peptides were studied in human lymph used as a model of tissue fluid. Both, classical and alternative pathways could be activated by suitable agents such as immune aggregates or zymosan. C3 activation and C3a formation were marked while only 10-15% of the anyway low amount of C5 were converted during complement activation, yielding very low concentrations of C5a. Carboxypeptidase N activity was present in lymph and converted the peptides to their less (C5a) or not at all (C3a) active desArg derivatives. Contact activation of Hageman factor and kallikrein enhanced activation of the classical pathway up to C3 conversion. The search for additional processes apt to create efficient concentrations of C5a (desArg) in lymph led to the discovery that the presence of leukocytes in lymph greatly increases the release of C5a activity upon complement activation. This suggests a physiological role of leukocytes resident in tissues for the induction of inflammatory reactions.
以人体淋巴液作为组织液模型，对补体成分、其激活过程以及C3a和C5a肽的生成进行了研究。经典途径和替代途径均可被免疫聚集体或酵母聚糖等合适的试剂激活。C3激活和C3a形成明显，而在补体激活过程中，无论C5含量本来就低，只有10% - 15%的C5被转化，产生的C5a浓度极低。淋巴液中存在羧肽酶N活性，可将这些肽转化为活性较低的（C5a）或完全无活性的（C3a）去精氨酸衍生物。Hageman因子和激肽释放酶的接触激活增强了经典途径直至C3转化的激活。对易于在淋巴液中产生有效浓度C5a（去精氨酸）的其他过程的研究发现，淋巴液中白细胞的存在会大大增加补体激活时C5a活性的释放。这表明组织中驻留的白细胞在诱导炎症反应中具有生理作用。
