Hartman C T, Glovsky M M
Int Arch Allergy Appl Immunol. 1981;66(3):274-81. doi: 10.1159/000232831.
The activation of human sera for histamine release by zymosan and anti-BSA-BSA complexes at equivalence was dose dependent and associated with a marked fall in CH50, C3 and C5 hemolytic activities. Heat-aggregated IgG, though able to fix greater than 90% of human CH50 activity and produce a lesser fall in C3 and C5 activities than zymosan and BSA-anti-BSA complexes, was unable to induce basophil histamine release. Chemically purified human C3a and C5a each caused histamine release from human basophils; C5a was more potent than C3a. In addition, neither zymosan nor BSA-anti-BSA complexes released histamine when incubated with homozygous C3-deficient serum. The addition of C3a and C5a at suboptimal concentrations produced an additive effect of histamine release.
酵母聚糖和等当量的抗牛血清白蛋白-牛血清白蛋白复合物激活人血清以释放组胺呈剂量依赖性，并伴有CH50、C3和C5溶血活性显著下降。热聚集的IgG虽然能够固定超过90%的人CH50活性，并且与酵母聚糖和牛血清白蛋白-抗牛血清白蛋白复合物相比，C3和C5活性下降较小，但不能诱导嗜碱性粒细胞组胺释放。化学纯化的人C3a和C5a均可引起人嗜碱性粒细胞组胺释放；C5a比C3a更有效。此外，酵母聚糖和牛血清白蛋白-抗牛血清白蛋白复合物与纯合C3缺陷血清孵育时均不释放组胺。以次优浓度添加C3a和C5a可产生组胺释放的相加效应。
