Department of Ceramic Engineering, National Institute of Technology Rourkela, Odisha, 769008, India.
Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha, 769008, India.
J Mech Behav Biomed Mater. 2023 Jan;137:105524. doi: 10.1016/j.jmbbm.2022.105524. Epub 2022 Oct 20.
This work is dedicated to combining nanotechnology with bone tissue engineering to prepare and characterize electrospun gelatin/monetite nanofibrous scaffold with improved physicochemical, mechanical, and biological properties. Nanofibrous scaffolds possessing fiber diameter in the range of 242-290 nm were prepared after incorporating varying content of monetite nanoparticles up to 7 wt % into the gelatin matrix using the electrospinning technique. Cross-linking of gelatin chains in the scaffold was performed using 0.25 wt% glutaraldehyde as indicated by imine (-CN-) bond formation in the FTIR analysis. With an increase in monetite addition up to 7 wt%, a decrease in swelling ratio and bio-degradability of cross-linked gelatin scaffolds was observed. Gelatin scaffold with 7 wt% monetite content registered the highest values of tensile strength and tensile modulus of 18.8 MPa and 170 MPa, as compared to 0% and 5 wt% monetite containing scaffolds respectively. Cell viability and differentiation were studied after culturing MG-63 cells onto the scaffolds from confocal microscopy of live and dead cells images, MTT assay, and alkaline phosphatase assay for a cell culture period of up to 21 days. It was observed that 7 wt % monetite containing gelatin scaffold exhibited better MG-63 cell adhesion, proliferation, higher biomineralization, and ALP activity compared to 0% and 5 wt% monetite containing electrospun scaffolds studied here.
这项工作致力于将纳米技术与骨组织工程相结合，制备和表征具有改善的物理化学、机械和生物性能的电纺明胶/羟磷灰石纳米纤维支架。通过电纺技术，将不同含量的羟磷灰石纳米颗粒（高达 7wt%）掺入明胶基质中，制备出纤维直径在 242-290nm 范围内的纳米纤维支架。交联明胶链在支架中使用 0.25wt%戊二醛进行，如在 FTIR 分析中形成的亚胺（-CN-）键所示。随着羟磷灰石添加量增加到 7wt%，交联明胶支架的溶胀率和生物降解性降低。与 0%和 5wt%羟磷灰石含量的支架相比，含有 7wt%羟磷灰石的明胶支架的拉伸强度和拉伸模量最高，分别为 18.8MPa 和 170MPa。在长达 21 天的细胞培养期间，通过共聚焦显微镜观察活细胞和死细胞图像、MTT 测定和碱性磷酸酶测定研究了细胞活力和分化。观察到含有 7wt%羟磷灰石的明胶支架与研究中的含有 0%和 5wt%羟磷灰石的电纺支架相比，具有更好的 MG-63 细胞黏附、增殖、更高的生物矿化和 ALP 活性。
