Ataabadi Mahmoud Sadeghi, Bahmanpour Soghra, Yousefinejad Saeed, Alaee Sanaz
Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz Univcersity of Medical Sciences, Shiraz, Iran.
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
J Steroid Biochem Mol Biol. 2023 Feb;226:106215. doi: 10.1016/j.jsbmb.2022.106215. Epub 2022 Nov 2.
Polycystic ovary syndrome (PCOS) as a common metabolic and endocrinological disorder can affect the metabolic profile in biological fluids. We studied the profile of blood volatile organic compounds (VOCs) in rats with PCOS and controls to identify potential specific biomarkers of blood VOCs in PCOS rats. For this purpose, 30 female adult Wistar rats were assigned to two groups: control and PCOS groups. PCOS model was induced using letrozole gavage (1 mg/kg) for 21 days. The rats in the control group received water of the same volume for 21 days. During treatment, a collection of vaginal smears was done every day for estrus cycle determination and weight was measured weekly. On the day after the last administration of letrozole, the rats were killed and their blood and ovaries were collected. Testosterone levels and histologic changes in ovaries were examined. Also, headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) analyzed the VOCs in the blood of PCOS and control rats. Multivariate and univariate statistical analyses were used to find the potential biomarkers for a rat model of PCOS. Weight gain, ovarian and vaginal pathological alteration, as well as hyperandrogenemia, confirmed the successful induction of the PCOS in rats. The results of blood VOCs analysis showed that nine VOCs were significantly elevated and one VOC decreased in the PCOS group than the control group (P < 0/05). The partial least-squares discriminant analysis (PLS-DA) and principal component analysis (PCA) showed good separation of VOCs between the PCOS rats and the control group. The 4-ethylphenol and capric (decanoic) acid were selected as the potential biomarkers for PCOS diagnosis in the blood of the PCOS rats. The blood of PCOS rats had a specific profile of VOCs, which could be detected by GC-MS analysis. These findings can pave the way for further studies towards developing a new screening method for PCOS detection and studying their pathology, based on VOCs analysis.
多囊卵巢综合征（PCOS）作为一种常见的代谢和内分泌紊乱疾病，会影响生物体液中的代谢特征。我们研究了PCOS大鼠和对照大鼠血液中挥发性有机化合物（VOCs）的特征，以确定PCOS大鼠血液VOCs的潜在特异性生物标志物。为此，将30只成年雌性Wistar大鼠分为两组：对照组和PCOS组。使用来曲唑灌胃（1mg/kg）诱导PCOS模型21天。对照组大鼠接受相同体积的水21天。治疗期间，每天采集阴道涂片以确定发情周期，并每周测量体重。在最后一次给予来曲唑后的第二天，处死大鼠并采集其血液和卵巢。检测睾酮水平和卵巢组织学变化。此外，顶空固相微萃取-气相色谱-质谱联用（HS-SPME-GC-MS）分析了PCOS大鼠和对照大鼠血液中的VOCs。采用多变量和单变量统计分析来寻找PCOS大鼠模型的潜在生物标志物。体重增加、卵巢和阴道病理改变以及高雄激素血症证实了大鼠PCOS模型诱导成功。血液VOCs分析结果显示，与对照组相比，PCOS组有9种VOCs显著升高，1种VOC降低（P< 0.05）。偏最小二乘判别分析（PLS-DA）和主成分分析（PCA）显示PCOS大鼠和对照组之间VOCs有良好的分离。4-乙基苯酚和癸酸被选为PCOS大鼠血液中PCOS诊断的潜在生物标志物。PCOS大鼠的血液具有特定的VOCs特征，可通过GC-MS分析检测到。这些发现可为基于VOCs分析开发PCOS检测新筛查方法及其病理学研究的进一步研究铺平道路。
