School of Environment and Energy, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, South China University of Technology, Guangzhou, Guangdong 510006, China.
School of Environment and Energy, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, South China University of Technology, Guangzhou, Guangdong 510006, China; School of Environment, South China Normal University, University Town, Guangzhou, Guangdong 510006, China.
Bioresour Technol. 2022 Dec;366:128227. doi: 10.1016/j.biortech.2022.128227. Epub 2022 Nov 1.
Sludge hydrolysis is a vital step in anaerobic digestion of sludge. This study compared the efficacy of free versus immobilized enzymes at different concentrations in promoting sludge disintegration. Pretreatment with 1,000 mg/L immobilized enzymes was more efficient in promoting sludge disintegration than free enzymes at the same concentration. Under the optimized conditions, volatile fatty acids (VFAs) were produced at 10.6 g/L, accounting for 85 % of total soluble chemical oxygen demand. Improved VFA production was attributed to the release of large amounts of polysaccharides and proteins from the enzymatically pretreated sludge. Released organic matter are the substrates for VFAs generated by the determined microbial community of Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, and Chloroflexi. In this study, anaerobic fermentation was used to successfully convert organic matter in sludge into high-value-added VFAs. Therefore, this process can be selected as a strategy to reduce carbon emissions from wastewater treatment plants (WWTPs).
污泥水解是污泥厌氧消化的重要步骤。本研究比较了不同浓度的游离酶和固定化酶在促进污泥解体方面的效果。在相同浓度下，用 1000mg/L 的固定化酶预处理比游离酶更能有效地促进污泥解体。在优化条件下，挥发性脂肪酸（VFAs）的产量为 10.6g/L，占总可溶化学需氧量的 85%。VFAs 产量的提高归因于大量多糖和蛋白质从酶预处理污泥中释放出来。释放出的有机物是由厚壁菌门、变形菌门、拟杆菌门、放线菌门和绿弯菌门等确定的微生物群落产生的 VFAs 的底物。在本研究中，采用厌氧发酵成功地将污泥中的有机物转化为高附加值的 VFAs。因此，该过程可作为减少污水处理厂（WWTP）碳排放的一种策略。
