Department of Environmental Science and Engineering, Huaqiao University, Xiamen 361021, China; Xiamen Engineering Research Center of Industrial Wastewater Biochemical Treatment, Xiamen 361021, China; Fujian Provincial Research Center of Industrial Wastewater Biochemical Treatment (Huaqiao University), Xiamen 361021, China.
Department of Environmental Science and Engineering, Huaqiao University, Xiamen 361021, China; Xiamen Engineering Research Center of Industrial Wastewater Biochemical Treatment, Xiamen 361021, China; Fujian Provincial Research Center of Industrial Wastewater Biochemical Treatment (Huaqiao University), Xiamen 361021, China.
Bioresour Technol. 2020 Apr;302:122869. doi: 10.1016/j.biortech.2020.122869. Epub 2020 Jan 23.
This study investigated the association of volatile fatty acid (VFA) production with microbial mechanism in waste activated sludge (WAS) anaerobic acidogenesis enhanced by freezing/thawing (F/T) pretreatment. WAS solubilization was enhanced with 955.4 ± 10.0 mg/L soluble chemical oxygen demand (SCOD) release by a 50-h F/T pretreatment at -24 °C. The highest level of VFAs (4852 ± 156 mg COD/L) was obtained after a 12-day fermentation. Moreover, phyla of Proteobacteria, Bacteroidetes, Firmicutes, and Ignavibacteriae played vital roles in VFA generation, while high genera abundance of Clostridium, Macellibacteroides, Prevotella, and Megasphaera were positively associated with high yields of short-chain (C2-C5) fatty acids. A schematic diagram was drawn to illustrate the microbial mechanism of enhanced VFA generation by F/T pretreatment during WAS fermentation. This study provides an in-depth exploration of promoting bio-resource recycling from WAS with a low-cost approach (specially in high latitudes) and bring about some new thinking on future WAS management.
本研究考察了在冻结/解冻（F/T）预处理增强的废活性污泥（WAS）厌氧产酸过程中，挥发性脂肪酸（VFA）生成与微生物机制的关系。通过在-24°C 下进行 50 小时的 F/T 预处理，WAS 的溶解率提高了 955.4±10.0mg/L 的可溶化学需氧量（SCOD）释放。经过 12 天的发酵，获得了最高水平的 VFAs（4852±156mg COD/L）。此外，变形菌门、拟杆菌门、厚壁菌门和Ignavibacteriae 在 VFA 生成中发挥了重要作用，而梭菌属、马塞拉氏菌属、普雷沃氏菌属和巨球形菌属等高丰度属与短链（C2-C5）脂肪酸的高产呈正相关。绘制了一个示意图，以说明 F/T 预处理在 WAS 发酵过程中增强 VFA 生成的微生物机制。本研究深入探讨了用低成本方法（特别是在高纬度地区）促进 WAS 的生物资源回收，并为未来的 WAS 管理带来了一些新的思路。
