从益生元衍生的生山羊奶中分离出的乳酸杆菌属：益生菌特性、胆固醇同化作用和叶酸生成
Lactobacillus spp. isolated from prebiotic-derived raw goat milk: probiotic characteristics, cholesterol assimilation and folate production.
作者信息
Tulumoğlu Şener, Kariptaş Ergin, Erdem Belgin
机构信息
Dr.Behçet Uz Hospital for Child Diseases Education and Research, Alsancak, 35210, İzmir, Turkey.
Faculty of Medicine, Department of Medical Microbiology, Samsun University, 55080, Samsun, Turkey.
出版信息
Biotechnol Lett. 2023 Jan;45(1):47-56. doi: 10.1007/s10529-022-03314-2. Epub 2022 Nov 4.
OBJECTIVES
This work provides to evaluate cholesterol assimilation and folic acid production by determining the probiotic properties of Lactobacillus spp. from raw goat milk with prebiotic properties.
RESULTS
We isolated Lactobacilli from goat milk and identified API 50, CHL, and 16sRNA. Probiotic properties were determined according to bile salt and acidic tolerance, hydrophobicity, hemolytic activity, antibiotic sensitivity, antagonistic effect, and exopolysaccharide production. In addition, the cholesterol assimilation and folate production of cultures were determined.
CONCLUSIONS
L. plantarum GM-12 and L. plantarum GM-15 showed the highest folate production and the highest cholesterol assimilation.These two strains are strong candidates for use as potential probiotics and starter cultures.
目的
本研究旨在通过测定具有益生元特性的生山羊奶中乳酸杆菌属的益生菌特性，评估胆固醇同化作用和叶酸生成情况。
结果
我们从山羊奶中分离出乳酸杆菌，并通过API 50、CHL和16sRNA进行鉴定。根据胆汁盐耐受性、酸耐受性、疏水性、溶血活性、抗生素敏感性、拮抗作用和胞外多糖生成情况来确定益生菌特性。此外，还测定了培养物的胆固醇同化作用和叶酸生成情况。
结论
植物乳杆菌GM - 12和植物乳杆菌GM - 15表现出最高的叶酸生成量和最高的胆固醇同化作用。这两株菌株是用作潜在益生菌和发酵剂的有力候选菌株。
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