使用重组 Fab 型抗体的基质金属蛋白酶 9 的快速酶联免疫吸附测定法。
A rapid ELISA for the detection of matrix metallopeptidase 9 using a recombinant Fab-type antibody.
机构信息
Department of Biological and Chemical Engineering, Hongik University, Sejong, 30016, Republic of Korea.
BioMAX/N-Bio Institute, Institute of Bioengineering, Seoul National University, Seoul, 08826, Republic of Korea.
出版信息
Biochem Biophys Res Commun. 2022 Dec 25;636(Pt 1):184-189. doi: 10.1016/j.bbrc.2022.10.087. Epub 2022 Oct 28.
Matrix metalloproteinase 9 (MMP9) contributes to several aspects of inflammation and cancer pathology, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant fragment antigen-binding (Fab)-type anti-MMP9 antibody in Escherichia coli with high purity within five days and confirmed the nanomolar order of antigen-binding efficiency of the recombinant Fab. Moreover, we optimized the experimental time for performing enzyme-linked immunosorbent assay (ELISA), and decreased the reaction time from the conventional 20.5 h to 3.5 h. The rapid and sensitive MMP9 detection system developed in this study can be applied to a range of applications, including the diagnosis of diseases with MMP9 overexpression including inflammatory and cancer-related diseases.
基质金属蛋白酶 9（MMP9）在炎症和癌症病理的多个方面发挥作用，包括侵袭、转移和血管生成。在这项研究中，我们在大肠杆菌中表达了一种重组片段抗原结合（Fab）型抗 MMP9 抗体，在五天内获得了高纯度的产物，并证实了重组 Fab 的抗原结合效率达到纳摩尔级。此外，我们还优化了进行酶联免疫吸附测定（ELISA）的实验时间，将反应时间从传统的 20.5 小时缩短至 3.5 小时。本研究开发的快速灵敏的 MMP9 检测系统可应用于多种领域，包括 MMP9 过表达相关疾病的诊断，如炎症和癌症相关疾病。
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