利用. 生产针对 MMP9 的可溶性重组抗体片段
Production of a Soluble Recombinant Antibody Fragment against MMP9 Using .
机构信息
Department of Biological and Chemical Engineering, Hongik University, Sejong 30016, Korea.
出版信息
Medicina (Kaunas). 2021 Sep 18;57(9):981. doi: 10.3390/medicina57090981.
Abstract
Matrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using , purified it, and confirmed its antigen-binding ability. The convenient, rapid, inexpressive system used in this study for producing recombinant antibody fragments needs only five days, and thus can be used for the efficient production of scFv against MMP9, which can be used in a range of applications and industrial fields, including diagnosis and treatment of inflammatory and cancer-related diseases.
摘要
基质金属蛋白酶 9（MMP9）参与癌症病理的多个方面，包括侵袭、转移和血管生成。在这项研究中，我们使用 表达了可溶性形式的重组 scFv 型抗 MMP9 抗体，对其进行了纯化，并证实了其抗原结合能力。本研究中使用的这种方便、快速、经济的表达系统生产重组抗体片段仅需 5 天，因此可用于高效生产针对 MMP9 的 scFv，可应用于诊断和治疗炎症和癌症相关疾病等一系列应用和工业领域。
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