College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Staff Development Institute of China National Tobacco Corporation, Zhengzhou 450000, China.
Bioresour Technol. 2023 Jan;367:128253. doi: 10.1016/j.biortech.2022.128253. Epub 2022 Nov 2.
Improper disposal of antibiotic fermentation dregs poses a risk of releasing antibiotics and antibiotic resistant bacteria to the environment. Therefore, this study evaluated the effects of biochar addition to lincomycin fermentation dregs (LFDs) composting. Biochar increased compost temperature and enhanced organic matter decomposition and residual antibiotics removal. Moreover, a 1.5- to 17.0-fold reduction in antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) was observed. Adding biochar also reduced the abundances of persistent ARGs hosts (e.g., Streptomyces, Pseudomonas) and ARG-related metabolic pathways and genes (e.g., ATP-binding cassette type-2 transport, signal transduction and multidrug efflux pump genes). By contrast, compost decomposition improved due to enhanced metabolism of carbohydrates and amino acids. Overall, adding biochar into LFDs compost reduced the proliferation of ARGs and enhanced microbial community metabolism. These results demonstrate that adding biochar to LFDs compost is a simple and efficient way to decrease risks associated with LFDs composting.
抗生素发酵废渣如果处理不当，会有抗生素和耐药菌释放到环境中的风险。因此，本研究评估了生物炭添加到林可霉素发酵废渣（LFDs）堆肥中的效果。生物炭增加了堆肥温度，促进了有机物分解和残留抗生素的去除。此外，抗生素耐药基因（ARGs）和可移动遗传元件（MGEs）的数量减少了 1.5 到 17.0 倍。添加生物炭还降低了持久性 ARG 宿主（如链霉菌属、假单胞菌属）和 ARG 相关代谢途径和基因（如 ATP 结合盒式 2 型转运蛋白、信号转导和多药外排泵基因）的丰度。相比之下，由于碳水化合物和氨基酸代谢的增强，堆肥的分解得到了改善。总的来说，将生物炭添加到 LFDs 堆肥中可以减少 ARGs 的增殖，增强微生物群落的代谢。这些结果表明，将生物炭添加到 LFDs 堆肥中是一种简单有效的降低 LFDs 堆肥风险的方法。
