College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
Bioresour Technol. 2022 Jul;355:127236. doi: 10.1016/j.biortech.2022.127236. Epub 2022 Apr 27.
Plant-derived and animal manure-derived biochars have been used to improve the quality of compost but the differences in their effects on antibiotic resistance genes (ARGs) during composting are unclear. This study selected two types of biochar (RB and PB) produced from abundant agricultural waste to be added to the compost. Adding plant-derived RB performed better in ARGs, mobile genetic elements, and human pathogenic bacteria removal during aerobic composting, whereas adding manure-derived PB even increased ARGs abundance. Vertical gene transfer was possibly the key mechanism for persistent ARGs, and easily removed ARGs were regulated by horizontal and vertical gene transfer. Adding plant-derived RB reduced the abundances of persistent ARG hosts (e.g., Pseudomonas and Longispora) and ARG-related metabolic pathways and genes. The higher nitrogen content of manure-derived PB may have promoted the proliferation of ARG hosts. Overall, adding manure-derived biochar during composting may not be the optimal option for eliminating ARGs.
植物源和动物源生物炭已被用于改善堆肥质量，但它们在堆肥过程中对抗生素抗性基因（ARGs）的影响尚不清楚。本研究选择了两种由丰富的农业废弃物制成的生物炭（RB 和 PB）添加到堆肥中。在好氧堆肥过程中，添加植物源 RB 对抗生素抗性基因、移动遗传元件和人病原细菌的去除效果更好，而添加粪源 PB 甚至会增加 ARGs 的丰度。垂直基因转移可能是持久 ARGs 的关键机制，而易去除的 ARGs 受水平和垂直基因转移的调节。添加植物源 RB 降低了持久性 ARG 宿主（如假单胞菌和长孢菌）和 ARG 相关代谢途径和基因的丰度。粪源 PB 较高的氮含量可能促进了 ARG 宿主的增殖。总的来说，在堆肥过程中添加粪源生物炭可能不是消除 ARGs 的最佳选择。
