Department of Pediatrics, The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, Huai'an City, Jiangsu Province, China.
Department of Digestive System, Jiangxi Provincial Children's Hospital, Nanchang, Jiangxi Province, China.
Allergol Immunopathol (Madr). 2022 Nov 1;50(6):147-153. doi: 10.15586/aei.v50i6.743. eCollection 2022.
To investigate the therapeutic effect of urolithin A (UA) on pediatric pneumonia and the underlying mechanisms.
The pediatric infantile pneumonia model was constructed by intratracheal induction of lipopolysaccharide (LPS) in 1-week-old C57BL/6 mice (male, 4-5 g). UA was also injected intraperitoneally. Lung tissues in each group were examined by histological analysis. Autophagy, inflammation, and oxidative stress were assessed by enzyme-linked--immunosorbent serologic assay and immunoblot analysis. Moreover, pyrophosis and endoplasmic reticulum stress were also evaluated by immunoblot analysis.
UA alleviated lung inflammation in mice, and inhibited cell pyrophosis. In addition, UA A relieved both oxidative and endoplasmic reticulum stress. Furthermore, we found that UA alleviated pneumonia damage by inducing protective autophagy.
UA induced protective autophagy to alleviate inflammation, oxidative stress, and endoplasmic reticulum stress in pediatric pneumonia.
研究尿石素 A（UA）对小儿肺炎的治疗作用及其机制。
通过气管内注入脂多糖（LPS）构建 1 周龄 C57BL/6 雄性幼鼠（4-5g）肺炎模型，腹腔内注射 UA。通过组织学分析检查各组肺组织。通过酶联免疫吸附试验和免疫印迹分析评估自噬、炎症和氧化应激。此外，还通过免疫印迹分析评估焦亡和内质网应激。
UA 减轻了小鼠肺部炎症，抑制了细胞焦亡。此外，UA 还减轻了氧化应激和内质网应激。进一步研究发现，UA 通过诱导保护性自噬来减轻肺炎损伤。
UA 通过诱导保护性自噬减轻小儿肺炎的炎症、氧化应激和内质网应激。
