Mozafari Atena, Amani Jafar, Shahsavandi Shahla, Hatef Salmanian Ali
Department of Agricultural Biotechnology. National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Biotechnol. 2022 Apr 1;20(2):e3119. doi: 10.30498/ijb.2022.298822.3119. eCollection 2022 Apr.
Newcastle disease, is one of the most important illnesses in the aviculture industry which shows a constant threat. In this case, the vaccine could be considered an important solution to prevent and control this disease. So, the development of a new and more effective vaccine against Newcastle disease is an urgent need. Immune informatics is an important field that provides insight into the experimental procedure and could facilitate the analysis of large amounts of immunological data generated by experimental research and help to design a new vaccine candidate.
This study is aimed at bioinformatics to investigate and select the most immunogenic and conserved epitopes derived from F and HN glycoproteins, which play a key role in pathogenesis and immunity. This strategy could cover a wide range of Newcastle disease viruses.
For expression in both (as an injectable recombinant vaccine candidate) and maize plant (as an edible vaccine candidate) host, two constructs were designed and analyzed separately. Furthermore, the role of LTB as an effective bio-adjuvant for general eliciting of the immune system and simultaneous expressions with those two antigens was evaluated. Hence, here a multimeric recombinant protein with the abbreviation LHN2F from the highly immunogenic part of HN, F and LTB proteins were designed. The synthetic construct was analyzed based on different bioinformatics tools.
The proper immunogenicity and stability of this multimeric fusion protein have been shown by immunoinformatic methods from various servers. To confirm the function of the designed protein, the final molecule was docked to chicken MHC class I using the Pyrex-python 0.8 program. the results of Immune Epitope analysis were confirmed by the docking results between protein and receptor.
The results of structural and immunological computational studies proposed that the protein deduced from this novel construct could act as a vaccine candidate for Newcastle disease virus control and prophylactic.
新城疫是养禽业中最重要的疾病之一，始终构成威胁。在此情况下，疫苗可被视为预防和控制该疾病的重要解决方案。因此，开发一种新型且更有效的抗新城疫疫苗迫在眉睫。免疫信息学是一个重要领域，它能深入了解实验过程，并有助于分析实验研究产生的大量免疫学数据，还能助力设计新的候选疫苗。
本研究旨在通过生物信息学方法，研究并筛选出F和HN糖蛋白中最具免疫原性且保守的表位，这两种糖蛋白在发病机制和免疫过程中起关键作用。此策略可涵盖多种新城疫病毒。
为了在大肠杆菌（作为注射用重组疫苗候选物）和玉米植株（作为食用疫苗候选物）宿主中表达，分别设计并分析了两种构建体。此外，评估了LTB作为有效生物佐剂对免疫系统的普遍激发作用以及与这两种抗原的同时表达情况。因此，在此设计了一种来自HN、F和LTB蛋白高免疫原性部分的缩写为LHN2F的多聚体重组蛋白。基于不同的生物信息学工具对合成构建体进行了分析。
来自不同服务器的免疫信息学方法已表明这种多聚体融合蛋白具有适当的免疫原性和稳定性。为了确认所设计蛋白的功能，使用Pyrex - python 0.8程序将最终分子与鸡MHC I类分子进行对接。蛋白与受体之间的对接结果证实了免疫表位分析的结果。
结构和免疫计算研究结果表明，从这种新型构建体推导的蛋白可作为控制和预防新城疫病毒的候选疫苗。
