Denis-Robichaud J, Christophe M, Roy J-P, Buczinski S, Rousseau M, Villettaz Robichaud M, Dubuc J
Independent researcher, Amqui, Québec, Canada, G5J 2N5.
Département de sciences cliniques, Faculté de médecine vétérinaire, Université de Montréal, 3200, rue Sicotte, St-Hyacinthe, QC, Canada, J2S 2M2.
JDS Commun. 2021 Aug 20;2(6):398-402. doi: 10.3168/jdsc.2021-0137. eCollection 2021 Nov.
The main objective of this study was to quantify the effect of pegbovigrastim (PEG) as an adjunct therapy for naturally occurring severe mastitis cases on survival, intramammary bacteriological cure, and subsequent milk production. A double-blinded, randomized controlled trial was conducted on a single commercial dairy farm. During the study period, all cows having a case of severe clinical mastitis, defined as the presence of abnormal milk and inflammation in one quarter or more combined with the presence of systemic signs (pyrexia, dehydration, or recumbency), were enrolled in the study. In addition to a standardized therapy combining systemic and intramammary antimicrobials as well as systemic anti-inflammatory drugs, cows received a subcutaneous injection of either 2.7 mL of 0.9% sterile saline (control group; CON) or 2.7 mL of PEG (PEG group). A milk sample for bacteriological analysis was taken before treatment was administered and a second sample was taken 14 d after enrollment. Survival (30 d post-treatment) and bacteriological cure (14 d post-treatment) were analyzed by survival analysis and chi-squared tests, respectively, whereas daily milk production was treated as a repeated measure in mixed regression models. The 77 cows enrolled in the study were of parity 2 to 4 (mean = 3) and between 3 and 302 d in milk (mean = 154). The bacteria identified in milk at enrollment were spp. (n = 48; 62%), (n = 16; 21%), spp. (n = 10; 13%), or no growth (n = 3; 4%). The probability of survival during the first 30 d after treatment was higher in the PEG group (84%) than in the control group (46%). Daily milk production over the 30-d period following treatment and bacteriological cure 14 d post-treatment did not differ between groups. Overall, cows treated with PEG as an adjunct therapy for naturally occurring cases of severe clinical mastitis had a better survival than cows treated with saline.
本研究的主要目的是量化培戈维司亭（PEG）作为自然发生的严重乳腺炎病例辅助治疗对奶牛生存、乳房内细菌学治愈及后续产奶量的影响。在一个商业奶牛场进行了一项双盲随机对照试验。在研究期间，所有患有严重临床乳腺炎的奶牛被纳入研究，严重临床乳腺炎定义为一个或多个乳区出现异常乳汁和炎症，并伴有全身症状（发热、脱水或卧地不起）。除了采用全身性和乳房内抗菌药物以及全身性抗炎药物联合的标准化治疗外，奶牛还皮下注射2.7 mL 0.9%无菌生理盐水（对照组；CON）或2.7 mL PEG（PEG组）。在给药前采集一份用于细菌学分析的乳样，并在入组后14天采集第二份乳样。分别通过生存分析和卡方检验分析生存情况（治疗后30天）和细菌学治愈情况（治疗后14天），而日泌乳量在混合回归模型中作为重复测量指标进行处理。纳入研究的77头奶牛胎次为2至4胎（平均 = 3），泌乳天数在3至302天之间（平均 = 154天）。入组时在乳汁中鉴定出的细菌为 spp.（n = 48；62%）、 （n = 16；21%）、 spp.（n = 10；13%）或无细菌生长（n = 3；4%）。PEG组治疗后前30天的生存概率（84%）高于对照组（46%）。治疗后30天期间的日泌乳量以及治疗后14天的细菌学治愈情况在两组之间无差异。总体而言，对于自然发生的严重临床乳腺炎病例，采用PEG作为辅助治疗的奶牛比采用生理盐水治疗的奶牛具有更好的生存情况。
