Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Pulawy, Poland.
J Vet Intern Med. 2024 Jan-Feb;38(1):505-513. doi: 10.1111/jvim.16939. Epub 2023 Dec 5.
Prevention of diseases in the early rearing of calves is important, particularly because disease occurrence most often requires antimicrobial administration but reduction of their use in animals is a priority. Pegbovigrastim is known for its use as an immunoregulator in cows and heifers, but the effect of its administration on calves has not been fully investigated.
Investigate whether administration of pegbovigrastim effectively stimulates nonspecific immunity in healthy calves.
Eleven clinically healthy 5-week-old calves.
Prospective observational study. Calves were randomly allocated to an experimental or control groups to receive pegbovigrastim or the same volume of phosphate-buffered saline twice over a 7-day period. To evaluate nonspecific immunity, the numbers of total leukocytes and cells in the appropriate cell fractions were determined. Cytometric analyses were carried out to identify cells expressing CD11b and to evaluate the phagocytic and oxidative burst activities of granulocytes and monocytes. Myeloperoxidase (MPO) and selected cytokines were assayed using ELISA.
Pegbovigrastim significantly increased the number of total leukocytes and of cells in all of the examined subsets (P < .05). The phagocytic activity of leukocytes expressed as mean fluorescence intensity was significantly potentiated after pegbovigrastim administration (P < .05). The cytokine response was modulated by pegbovigrastim administration toward anti-inflammatory activity.
Pegbovigrastim effectively stimulated nonspecific immunity in clinically healthy calves, which in the long term could make the prevention of diseases during early rearing possible by strengthening the immune defense mechanisms of the host.
预防犊牛早期疾病非常重要，特别是因为疾病发生通常需要使用抗菌药物，但减少动物抗菌药物的使用是当务之急。培格非格司亭作为一种免疫调节剂在奶牛和小母牛中已得到广泛应用，但它在犊牛中的应用效果尚未得到充分研究。
研究培格非格司亭是否能有效刺激健康犊牛的非特异性免疫。
11 头临床健康的 5 周龄犊牛。
前瞻性观察性研究。将犊牛随机分配到实验组或对照组，在 7 天内接受培格非格司亭或等量磷酸盐缓冲液两次。为评估非特异性免疫，测定总白细胞数和适当细胞群中的细胞数。通过流式细胞术分析鉴定表达 CD11b 的细胞，并评估粒细胞和单核细胞的吞噬和氧化爆发活性。使用 ELISA 测定髓过氧化物酶（MPO）和选定细胞因子。
培格非格司亭显著增加了总白细胞数和所有检查亚群中的细胞数（P < .05）。培格非格司亭给药后，白细胞的吞噬活性（以平均荧光强度表示）显著增强（P < .05）。细胞因子反应受培格非格司亭给药调节，向抗炎活性方向转变。
培格非格司亭能有效刺激临床健康犊牛的非特异性免疫，从长远来看，通过增强宿主的免疫防御机制，可能使早期饲养期间疾病的预防成为可能。
