Zhou Yongbing, Qiao Ying, Zhang Xuemin, Ma Xingzhuang, Liu Hui, Wang Li
School of Public Health, Bengbu Medical College, Bengbu 233030, PR China.
Department of Biochemistry and Molecular Biology, School of Laboratory Medicine, Anhui Province Key Laboratory of Immunology in Chronic Diseases, Bengbu Medical College, Bengbu 233030, PR China.
Toxicol Res (Camb). 2022 Oct 3;11(5):872-880. doi: 10.1093/toxres/tfac065. eCollection 2022 Oct.
Perfluorooctanoic acid (PFOA) is a type of 8-carbon perfluoroalkyl substances (PFASs) widely used in industrial and domestic products, which now is a persistent organic pollutant (POP) found in the environment. Its structure is similar to fatty acids, which enables it to induce the expression of ACOT genes. To investigate the expression levels of Acot1 in various tissues and organs after exposure to PFOA for 28 days in rats, and to compare the variations of Acot1 expression in different tissues, we sectioned samples and incubated with Acot1 antibody. The results show that the transcription and protein expression levels of Acot1 in the liver and kidney of rats increased significantly. Meanwhile, the transcription and protein expression of Acot1 gene were also detected in testis, muscle, and adipose. The results of immunohistochemistry were also verified by western blot detection, and we detected the transcription of Acot1 gene in these tissues and found that they all increased in varying degrees. In this study, the expression of Acot1 protein in rat serum was detected for the first time, and the expression of Acot1 in rat serum was found to be significantly increased after PFOA exposure. In addition, the expression level of Acot1 in rat organism was found to be higher than that in the control group after 4 days of depuration for 7 days of acute PFOA exposure, and Acot1 protein expression also showed an increase with increasing exposure time, indicating that Acot1 can be used as a sensitive biomarker for health monitoring of PFOA occupational workers or exposed persons.
全氟辛酸（PFOA）是一种含8个碳的全氟烷基物质（PFASs），广泛应用于工业和家庭产品中，现在是一种在环境中发现的持久性有机污染物（POP）。其结构与脂肪酸相似，这使其能够诱导ACOT基因的表达。为了研究大鼠暴露于PFOA 28天后各种组织和器官中Acot1的表达水平，并比较不同组织中Acot1表达的变化，我们对样本进行切片并与Acot1抗体孵育。结果表明，大鼠肝脏和肾脏中Acot1的转录和蛋白表达水平显著增加。同时，在睾丸、肌肉和脂肪中也检测到了Acot1基因的转录和蛋白表达。免疫组化结果也通过蛋白质印迹检测得到验证，我们检测了这些组织中Acot1基因的转录，发现它们均有不同程度的增加。在本研究中，首次检测了大鼠血清中Acot1蛋白的表达，发现PFOA暴露后大鼠血清中Acot1的表达显著增加。此外，急性PFOA暴露7天后净化4天，大鼠机体中Acot1的表达水平高于对照组，且Acot1蛋白表达也随暴露时间的增加而升高，表明Acot1可作为PFOA职业工人或暴露人群健康监测的敏感生物标志物。
