Živković Andrej, Gođevac Dejan, Cigić Blaž, Polak Tomaž, Požrl Tomaž
Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, SI-1111 Ljubljana, Slovenia.
Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia.
Foods. 2023 Apr 24;12(9):1769. doi: 10.3390/foods12091769.
In this study, we investigated the effects of germination on the secondary metabolite composition in spelt grains. Germination significantly increased the content of various metabolites in free and bound forms. Benzoxazinoids were the most important compounds in the free fraction of the 96 h germinated grains (MBOA content as the predominant compound was 277.61 ± 15.29 µg/g DW). The majority of phenolic acids were present in the bound fraction, with -ferulic acid as the main component, reaching 753.27 ± 95.87 µg/g DW. The often neglected -isomers of phenolic acids accounted for about 20% of the total phenolic acids. High levels of apigenin di-C-glycosides were found in spelt grains, and the schaftoside content was most affected by germination, increasing threefold. The accumulation of secondary metabolites significantly increased the antioxidant activity of germinated spelt. According to the results of this study, the content of most bioactive compounds was highest in spelt grains after 96 h of germination. These data suggest that germinated spelt could potentially be valuable for the production of functional foods.
在本研究中，我们调查了发芽对斯佩尔特小麦籽粒中次生代谢产物组成的影响。发芽显著增加了游离态和结合态各种代谢产物的含量。苯并恶嗪类化合物是96小时发芽籽粒游离部分中最重要的化合物（作为主要化合物的MBOA含量为277.61±15.29μg/g干重）。大多数酚酸存在于结合部分，其中阿魏酸为主要成分，含量达到753.27±95.87μg/g干重。酚酸中常被忽视的异构体约占总酚酸的20%。在斯佩尔特小麦籽粒中发现了高水平的芹菜素二碳糖苷，其中schaftoside含量受发芽影响最大，增加了两倍。次生代谢产物的积累显著提高了发芽斯佩尔特小麦的抗氧化活性。根据本研究结果，大多数生物活性化合物的含量在发芽96小时后的斯佩尔特小麦籽粒中最高。这些数据表明，发芽的斯佩尔特小麦可能对功能性食品的生产具有潜在价值。
