Wang Hong-Ling, Liu Yilin, Zhou Tiantian, Gao Lumin, Li Jianxi, Wu Xin, Yin Yu-Long
Hunan Co-Innovation Center of Safety Animal Production, College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation, Zhang Zhongjing College of Chinese Medicine, Nanyang Institute of Technology, Nanyang, China.
Front Nutr. 2022 Oct 20;9:1018349. doi: 10.3389/fnut.2022.1018349. eCollection 2022.
As an important nucleoside precursor in salvage synthesis pathway of uridine monophosphate, uridine (UR) is the most abundant nucleotide in sow milk. This study aimed to investigate the effects of maternal UR supplementation during second trimester of gestation on reproductive performance and amino acid metabolism of Sows.
Results showed that compared to CON group, the average number of stillborn piglets per litter was significantly reduced ( < 0.05) with higher average piglet weight at birth in UR group ( = 0.083). Besides, dietary UR supplementation significantly increased TP in sow serum, BUN content in cord serum, and TP and ALB in newborn piglet serum ( < 0.05); but decreased AST level in sow serum and BUN level in piglet serum ( < 0.05). Importantly, free amino acids profile in sow serum newborn piglet serum and colostrum was changed by maternal UR supplementation during day 60 of pregnancy, as well as the expression of amino acids transporter ( < 0.05). In addition, from 100 to 2,000 μM UR can increased the viability of pTr2 cells. The UR exhibited higher distribution of G1/M phase of cell cycle at 400 μM compared with 0 μM, and reduced S-phases of cell cycle compared with 0 and 100μM ( < 0.05).
Supplementation of uridine during day 60 of pregnancy can improve reproductive performance, regulate amino acid metabolism of sows and their offspring, and increase the viability of pTr2 cells.
尿苷（UR）作为单磷酸尿苷补救合成途径中的一种重要核苷前体，是母猪乳汁中含量最丰富的核苷酸。本研究旨在探讨妊娠中期母体补充UR对母猪繁殖性能和氨基酸代谢的影响。
结果表明，与对照组相比，UR组每窝死胎平均数量显著减少（<0.05），出生时仔猪平均体重更高（=0.083）。此外，日粮中添加UR显著提高了母猪血清总蛋白（TP）、脐带血清尿素氮（BUN）含量以及新生仔猪血清TP和白蛋白（ALB）水平（<0.05）；但降低了母猪血清天冬氨酸氨基转移酶（AST）水平和仔猪血清BUN水平（<0.05）。重要的是，妊娠第60天母体补充UR改变了母猪血清、新生仔猪血清和初乳中的游离氨基酸谱，以及氨基酸转运体的表达（<0.05）。此外，100至2000μM的UR可提高pTr2细胞的活力。与0μM相比，400μM的UR使细胞周期G1/M期分布更高，与0和100μM相比，细胞周期S期减少（<0.05）。
妊娠第60天补充尿苷可提高繁殖性能，调节母猪及其后代的氨基酸代谢，并提高pTr2细胞的活力。
