Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, China.
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China.
J Sci Food Agric. 2020 Jul;100(9):3709-3718. doi: 10.1002/jsfa.10410. Epub 2020 Apr 27.
Nucleotides play an important role in the regulation of cellular energy and protein homeostasis, which facilitate the repair, recovery and repletion of tissue function. This study tested the effects of maternal uridine (UR) supplementation during late pregnancy and lactation of sows on the immune function of the small intestine in neonatal and suckling piglets.
Results showed that compared to the control group, maternal dietary UR supplementation significantly decreased incidence of diarrhea in suckling piglets (P < 0.01); and increased both duodenal and ileal average villus height (P < 0.01) as well as villus height/crypt depth in ileum (P = 0.017) in neonatal piglets. RT-qPCR results showed that maternal UR supplementation decreased mRNA expression of claudin-1 in jejunum and ileum of neonatal piglets (P < 0.05), while significantly increased mRNA expression of claudin-1 in duodenum and jejunum of suckling piglets. Furthermore, in suckling piglets, maternal dietary UR supplementation increased mRNA expression of IL-6, IL-8 and IL-1β in duodenum, jejunum and ileum (P < 0.05), increased IL-10 expression in both jejunal and ileal mucosa (P < 0.05) and increased mRNA expression of IKB and TLR4 in ileal mucosa (P < 0.05).
These results suggest that maternal dietary supplementation with UR contributed to reducing incidence of diarrhea by regulating cytokine secretion and intestinal mucosal barrier function in suckling piglets. © 2020 Society of Chemical Industry.
核苷酸在调节细胞能量和蛋白质动态平衡方面发挥着重要作用，这有助于组织功能的修复、恢复和补充。本研究测试了在母猪妊娠晚期和哺乳期补充尿苷（UR）对新生和哺乳期仔猪小肠免疫功能的影响。
结果表明，与对照组相比，母鼠日粮 UR 补充显著降低了哺乳期仔猪腹泻的发生率（P<0.01）；并增加了新生仔猪十二指肠和回肠的平均绒毛高度（P<0.01）以及回肠的绒毛高度/隐窝深度（P=0.017）。RT-qPCR 结果显示，UR 补充降低了新生仔猪空肠和回肠中 Claudin-1 的 mRNA 表达（P<0.05），而显著增加了哺乳期仔猪空肠和回肠中 Claudin-1 的 mRNA 表达。此外，在哺乳期仔猪中，母鼠日粮 UR 补充增加了十二指肠、空肠和回肠中 IL-6、IL-8 和 IL-1β 的 mRNA 表达（P<0.05），增加了空肠和回肠黏膜中 IL-10 的表达（P<0.05），并增加了回肠黏膜中 IKB 和 TLR4 的 mRNA 表达（P<0.05）。
这些结果表明，母鼠日粮补充 UR 通过调节哺乳期仔猪细胞因子分泌和肠黏膜屏障功能，有助于降低腹泻发生率。 © 2020 英国化学学会。
