Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Medical Laboratory Sciences, School of Paramedical Sciences, Dezful University of Medical Sciences, Dezful, Iran.
Biomed Res Int. 2022 Oct 26;2022:2988334. doi: 10.1155/2022/2988334. eCollection 2022.
Liver damage occurs following renal ischemia-reperfusion (RIR) that can cause inflammation and inflammatory cytokines activated after kidney injury. In this study, thyme essential oil (TE) with antioxidant and anti-inflammatory properties was used to reduce liver damage induced by renal IR. 32 male rats were randomly divided into 4 equal groups: (1) control, (2) RIR, (3) RIR+TE, and (4) TE. Rats received TE as a pretreatment at a dose of 0.5 ml/kg for one week. Then, under anesthesia for 45 minutes for ischemia, the kidneys of the animals were closed with clamps, and reperfusion was performed for 24 hours. Animal serum was isolated to evaluate alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) parameters. The liver of rats was examined for the measurement of malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH), glutathione peroxidase (GPX), catalase (CAT), and expression of genes such as interleukin-6 (IL-6), tumor necrosis factor- (TNF-), and caspase-3. ALP, AST, ALT, MDA, NO, IL-6, TNF-, and caspase-3 increased significantly in the RIR group compared to the control group ( < 0.05). GSH, GPX, and CAT decreased significantly in the RIR group compared to the control group ( < 0.05). TE caused a decrease in ALP, AST, ALT, MDA, NO, IL-6, and TNF- compared to the RIR group and caused an increase in the amount of GSH, GPX, and CAT in the RIR group ( < 0.05). This study showed that TE has antioxidant and anti-inflammatory properties that reduce liver damage induced by RIR.
肾缺血再灌注（RIR）后会发生肝损伤，这可能导致肾脏损伤后炎症和炎症细胞因子的激活。在这项研究中，具有抗氧化和抗炎特性的百里香精油（TE）被用于减少肾 IR 引起的肝损伤。32 只雄性大鼠被随机分为 4 组：（1）对照组，（2）RIR 组，（3）RIR+TE 组和（4）TE 组。大鼠接受 TE 预处理，剂量为 0.5ml/kg，持续一周。然后，在麻醉下缺血 45 分钟，用夹具关闭动物的肾脏，并进行 24 小时再灌注。分离动物血清以评估碱性磷酸酶（ALP）、天门冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）参数。检查大鼠肝脏以测量丙二醛（MDA）、一氧化氮（NO）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPX）、过氧化氢酶（CAT）以及白细胞介素-6（IL-6）、肿瘤坏死因子-（TNF-）和半胱天冬酶-3 的基因表达。与对照组相比，RIR 组的 ALP、AST、ALT、MDA、NO、IL-6、TNF-和半胱天冬酶-3 显著增加（<0.05）。与对照组相比，RIR 组的 GSH、GPX 和 CAT 显著减少（<0.05）。与 RIR 组相比，TE 组的 ALP、AST、ALT、MDA、NO、IL-6 和 TNF- 减少，而 RIR 组的 GSH、GPX 和 CAT 增加（<0.05）。这项研究表明，TE 具有抗氧化和抗炎特性，可减轻 RIR 引起的肝损伤。
