Wang Peixin, Ma Xin, Zhang Riteng, Zhao Yongxin, Hu Ruochen, Luo Chen, Zeshan Basit, Yang Zengqi, Qiu Li, Wang Juan, Liu Haijin, Zhou Yefei, Wang Xinglong
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Sandakan, Sabah, Malaysia.
Front Microbiol. 2022 Oct 19;13:1022481. doi: 10.3389/fmicb.2022.1022481. eCollection 2022.
The widespread and endemic circulation of porcine reproductive and respiratory syndrome virus (PRRSV) cause persistent financial losses to the swine industry worldwide. In 2017, NADC34-like PRRSV-2 emerged in northeastern China and spread rapidly. The dynamics analysis of immune perturbations associated with novel PRRSV lineage is still incomplete. This study performed a time-course transcriptome sequencing of NADC34-like PRRSV strain YC-2020-infected porcine alveolar macrophages (PAMs) and compared them with JXA1-infected PAMs. The results illustrated dramatic changes in the host's differentially expressed genes (DEGs) presented at different timepoints after PRRSV infection, and the expression profile of YC-2020 group is distinct from that of JXA1 group. Functional enrichment analysis showed that the expression of many inflammatory cytokines was up-regulated following YC-2020 infection but at a significantly lower magnitude than JXA1 group, in line with the trends for most interferon-stimulated genes (ISGs) and their regulators. Meanwhile, numerous components of histocompatibility complex (MHC) class II and phagosome presented a stronger transcription suppression after the YC-2020 infection. All results imply that YC-2020 may induce milder inflammatory responses, weaker antiviral processes, and more severe disturbance of antigen processing and presentation compared with HP-PRRSV. Additionally, , , and which were selected from weighted gene co-expression network analysis (WGCNA), could significantly inhibit PRRSV proliferation. This study provides fundamental data for understanding the biological characteristics of NADC34-like PRRSV and new insights into PRRSV evolution and prevention.
猪繁殖与呼吸综合征病毒（PRRSV）的广泛流行和地方流行给全球养猪业造成了持续的经济损失。2017年，类似NADC34的PRRSV-2在中国东北地区出现并迅速传播。与新型PRRSV谱系相关的免疫扰动动态分析仍不完整。本研究对感染类似NADC34的PRRSV毒株YC-2020的猪肺泡巨噬细胞（PAM）进行了时间进程转录组测序，并将其与感染JXA1的PAM进行了比较。结果表明，PRRSV感染后不同时间点宿主差异表达基因（DEG）发生了显著变化，YC-2020组的表达谱与JXA1组不同。功能富集分析表明，YC-2020感染后许多炎性细胞因子的表达上调，但幅度明显低于JXA1组，这与大多数干扰素刺激基因（ISG）及其调节因子的趋势一致。同时，组织相容性复合体（MHC）II类和吞噬体的许多成分在YC-2020感染后呈现出更强的转录抑制。所有结果表明，与高致病性PRRSV相比，YC-2020可能诱导更温和的炎症反应、更弱的抗病毒过程以及更严重的抗原加工和呈递紊乱。此外，从加权基因共表达网络分析（WGCNA）中筛选出的 、 、 可显著抑制PRRSV增殖。本研究为了解类似NADC34的PRRSV的生物学特性提供了基础数据，并为PRRSV的进化和预防提供了新的见解。
