Phillips Carrie, Haldane David Jm
Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Provincial Public Health Laboratory Network, Halifax, Nova Scotia, Canada.
J Assoc Med Microbiol Infect Dis Can. 2021 Dec 3;6(4):307-312. doi: 10.3138/jammi-2020-0034. eCollection 2021 Dec.
The antimicrobial susceptibility of isolates of to agents used for chemoprophylaxis and to penicillin G was determined.
Fifty strains detected in Nova Scotia between 2004 and 2018 were included. The isolates were originally isolated from sites that might prompt chemoprophylaxis (27 blood, 18 cerebrospinal fluid [CSF], 3 CSF-blood, and 2 conjunctiva). Minimal inhibitory concentrations (MICs) were determined to azithromycin, ciprofloxacin, minocycline, rifampin, trimethoprim-sulfamethoxazole, and penicillin G, using a diffusion gradient strip on Mueller-Hinton agar with 5% sheep blood in 5% CO2 for 20-24 hours.
All isolates remained susceptible to azithromycin, ciprofloxacin, minocycline, and rifampin, but there was 26% resistance to trimethoprim-sulfamethoxazole. There was a rise in penicillin MIC of the isolates over the study period.
In our province, isolates causing invasive infection remain susceptible to commonly used antimicrobial agents used for chemoprophylaxis. Significant resistance to trimethoprim-sulfamethoxazole is present and the degree of susceptibility to penicillin G is lessening.
测定了分离株对用于化学预防的药物以及青霉素G的抗菌药敏性。
纳入2004年至2018年在新斯科舍省检测到的50株菌株。这些分离株最初是从可能需要化学预防的部位分离出来的（27份血液、18份脑脊液[CSF]、3份脑脊液-血液和2份结膜样本）。使用在含5%羊血的Mueller-Hinton琼脂上的扩散梯度条，在5%二氧化碳环境中培养20 - 24小时，测定对阿奇霉素、环丙沙星、米诺环素、利福平、甲氧苄啶-磺胺甲恶唑和青霉素G的最低抑菌浓度（MICs）。
所有分离株对阿奇霉素、环丙沙星、米诺环素和利福平仍敏感，但对甲氧苄啶-磺胺甲恶唑有26%的耐药率。在研究期间，分离株的青霉素MIC有所上升。
在我省，引起侵袭性感染的分离株对常用的化学预防抗菌药物仍敏感。对甲氧苄啶-磺胺甲恶唑存在显著耐药性，并且对青霉素G的敏感程度正在降低。
