人源环状RNA hsa_circ_0040809和hsa_circ_0000467促进结肠癌细胞进展并构建环状RNA-微小RNA-信使RNA网络。
Hsa_circ_0040809 and hsa_circ_0000467 promote colorectal cancer cells progression and construction of a circRNA-miRNA-mRNA network.
作者信息
Liu Jingfu, Chen Shan, Li Zhen, Teng Wenhao, Ye Xianren
机构信息
Department of Blood Transfusion, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China.
Department of Gastrointestinal Surgery, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China.
出版信息
Front Genet. 2022 Oct 20;13:993727. doi: 10.3389/fgene.2022.993727. eCollection 2022.
Circular RNAs (circRNAs) have been demonstrated to be closely involved in colorectal cancer (CRC) pathogenesis and metastasis. More potential biomarkers are needed to be searched for colorectal cancer (CRC) diagnosis and treatment. The objective of this study is to seek differentially expressed circRNAs (DEcircRNAs), test their roles in CRC and construct a potential competing endogenous RNA (ceRNA) network. CircRNA microarrays were obtained from Gene Expression Omnibus, and differential expression was analyzed by R software. The relative expressions of DEcircRNAs were confirmed in CRC tissues and cell lines by qRT-PCR. MTs and Transwell experiments were performed for detecting the roles of circRNAs on CRC cell proliferation and migration, respectively. Targeted miRNAs of circRNAs and targeted mRNAs of miRNAs were predicted and screened by bioinformatics methods. A ceRNA network of DEcircRNAs was constructed by Cytoscape. To further verify the potential ceRNA network, the expressions of miRNAs and mRNAs in knockdown of DEcircRNAs CRC cells were detected by qRT-PCR. Two DEcircRNAs (hsa_circ_0040809 and hsa_circ_0000467) were identified and validated in CRC tissues and cell lines. The results of MTs and Transwell experiments showed that hsa_circ_0040809 and hsa_circ_0000467 promoted CRC proliferation and migration. Bioinformatics analysis screened 3 miRNAs (miR-326, miR-330-5p, and miR-330-3p) and 2 mRNAs ( and ), and a ceRNA network was constructed. In knockdown of hsa_circ_0040809 HCT-116 cells, the expression of miR-330-3p was significantly upregulated, while was significantly downregulated. In knockdown of hsa_circ_0000467 HCT-116 cells, the expressions of miR-326 and miR-330-3p were upregulated, while was downregulated. We found that hsa_circ_0040809 and hsa_circ_0000467 were upregulated in CRC tissues and cell lines, and promoted CRC cell progression. A circRNA-miRNA-mRNA network based on hsa_circ_0040809 and hsa_circ_0000467 was constructed.
环状RNA（circRNAs）已被证明与结直肠癌（CRC）的发病机制和转移密切相关。需要寻找更多潜在的生物标志物用于结直肠癌（CRC）的诊断和治疗。本研究的目的是寻找差异表达的环状RNA（DEcircRNAs），测试它们在CRC中的作用，并构建一个潜在的竞争性内源RNA（ceRNA）网络。从基因表达综合数据库获取环状RNA微阵列，并通过R软件分析差异表达。通过qRT-PCR在CRC组织和细胞系中确认DEcircRNAs的相对表达。分别进行MTs和Transwell实验以检测环状RNA对CRC细胞增殖和迁移的作用。通过生物信息学方法预测和筛选环状RNA的靶向miRNA以及miRNA的靶向mRNA。用Cytoscape构建DEcircRNAs的ceRNA网络。为进一步验证潜在的ceRNA网络，通过qRT-PCR检测DEcircRNAs敲低的CRC细胞中miRNA和mRNA的表达。在CRC组织和细胞系中鉴定并验证了两个DEcircRNAs（hsa_circ_0040809和hsa_circ_0000467）。MTs和Transwell实验结果表明，hsa_circ_0040809和hsa_circ_0000467促进CRC增殖和迁移。生物信息学分析筛选出3个miRNA（miR-326、miR-330-5p和miR-330-3p）和2个mRNA（以及），并构建了一个ceRNA网络。在hsa_circ_0040809敲低的HCT-116细胞中，miR-330-3p的表达显著上调，而显著下调。在hsa_circ_0000467敲低的HCT-116细胞中，miR-326和miR-330-3p的表达上调，而下调。我们发现hsa_circ_0040809和hsa_circ_0000467在CRC组织和细胞系中上调，并促进CRC细胞进展。构建了基于hsa_circ_0040809和hsa_circ_0000467的circRNA-miRNA-mRNA网络。
Zotero 插件