Xia Xiaoyi, Wang Huilei, Duan Yelin, Yang Li, He Junwei
Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, China.
College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China.
Heliyon. 2022 Oct 22;8(11):e11176. doi: 10.1016/j.heliyon.2022.e11176. eCollection 2022 Nov.
(Steph. ex Willd.) Ikonn.-Gal. has been a traditional Chinese medicine (TCM) for the treatment of blood stasis, inflammation, and diuresis. Moreover, theory was an important TCM theory that used to treat many ailments. Nevertheless, the scientific connotation of this theory has not been clearly elucidated so far.
The aim of this study was to explore the scientific connotation of with promoting blood circulation and removing blood stasis (PBCRBS), anti-inflammatory and diuretic effects in trauma-induced blood stasis model (TBSM) rats, taking microporous adsorption resin with water (LSB) and 30% ethanol (LSC) elution fractions from as a classical demonstration.
48 rats were randomly assigned into six groups (n = 8/group): the control group, the model group, and model groups treatment with LSB or LSC. The biochemical parameters and protein expression were measured using kit method and Western blot assay, respectively.
Both LSB and LSC were effective in elevating body weight, food consumption, and water intake in model rats. In PBCRBS efficacy evaluation, LSB and LSC remarkably improved histopathological tissues. On the other hand, LSB and LSC prominently decreased the contents of plasma viscosity, platelet aggregation rate, thrombin time, prothrombin time, activated partial thromboplastin time (APTT), fibrinogen, thromboxane B2, thromboxane B2/6-keto-prostaglandin F1α, urokinase-type plasminogen activator (u-PA), plasminogen activator inhibitor-1(PAI-1), PAI-1/tissue-type plasminogen activator (t-PA), and PAI-1/u-PA, while significantly enhanced the contents of antithrombin III, 6-keto-prostaglandin F1α, and t-PA. In parallel, LSB and LSC obviously down-regulated the levels of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and IL-8, and remarkably up-regulated the level of IL-10. In determining diuretic activities, LSB and LSC prominently elevated urinary excretion volume and the level of atriopeptin, and remarkably reduced the levels of angiotensin II, anti-diuretic hormone, aldosterone, aquaporin 1 (AQP1), AQP2, and AQP3. In addition, LSB and LSC clearly suppressed protein expressions of AQP1, AQP2, and AQP3. Finally, LSB and LSC did not caused urinary pH, Na, and Cl electrolytes and had minor effects on K and Ca concentrations.
LSB and LSC exhibited prominent PBCRBS, anti-inflammatory, and diuretic effects in TBSM rats, thereby supported the traditional folk use of . This study successfully provided an experimental basis for the scientific connotation of .
（Steph. ex Willd.）Ikonn.-Gal. 一直是用于治疗血瘀、炎症和利尿的传统中药。此外，[理论名称]是用于治疗多种疾病的重要中医理论。然而，该理论的科学内涵迄今尚未得到明确阐释。
本研究旨在探讨[药物名称]在创伤性血瘀模型（TBSM）大鼠中活血化瘀、抗炎和利尿作用的科学内涵，以水（LSB）和30%乙醇（LSC）洗脱部位的微孔吸附树脂为经典示范。
48只大鼠随机分为六组（每组n = 8）：对照组、模型组以及用LSB或LSC治疗的模型组。分别采用试剂盒法和蛋白质印迹法测定生化参数和蛋白表达。
LSB和LSC均能有效提高模型大鼠的体重、食物摄入量和饮水量。在活血化瘀功效评价中，LSB和LSC显著改善了组织病理学表现。另一方面，LSB和LSC显著降低了血浆黏度、血小板聚集率、凝血酶时间、凝血酶原时间、活化部分凝血活酶时间（APTT）、纤维蛋白原、血栓素B2、血栓素B2/6-酮-前列腺素F1α、尿激酶型纤溶酶原激活剂（u-PA）、纤溶酶原激活剂抑制剂-1（PAI-1）、PAI-1/组织型纤溶酶原激活剂（t-PA）以及PAI-1/u-PA的含量，同时显著提高了抗凝血酶III、6-酮-前列腺素F1α和t-PA的含量。与此同时，LSB和LSC明显下调了肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）和IL-8的水平，并显著上调了IL-10的水平。在利尿活性测定中，LSB和LSC显著提高了尿排泄量和心钠素水平，并显著降低了血管紧张素II、抗利尿激素、醛固酮、水通道蛋白1（AQP1）、AQP2和AQP3的水平。此外，LSB和LSC明显抑制了AQP1、AQP2和AQP3的蛋白表达。最后，LSB和LSC未引起尿液pH值、钠和氯电解质变化，对钾和钙浓度影响较小。
LSB和LSC在TBSM大鼠中表现出显著的活血化瘀、抗炎和利尿作用，从而支持了[药物名称]的传统民间用途。本研究成功为[药物名称]的科学内涵提供了实验依据。
