Zhang Shengyuan, Nie Hua, Yang Yali, Yang Li, He Junwei
Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Aeras, Jiaying University, Meizhou, 514015, P. R. China.
Jiangxi University of Chinese Medicine, Nanchang, 330004, P. R. China.
Chem Biodivers. 2023 Mar;20(3):e202201176. doi: 10.1002/cbdv.202201176. Epub 2023 Feb 16.
Leonurus japonicus Houtt. has been traditionally used to treat many ailments. This study evaluated the activating blood circulation, anti-inflammatory, and diuretic effects of L. japonicus extract (LJ) and identified its phytochemicals. In this work, the phytochemicals in LJ were identified using liquid chromatography mass spectrometry. Rats were randomly assigned to three groups (n=8): Control group was treated with saline, while the Model group (saline) and LJ group (426 mg/kg) had induced traumatic injury. All rats were treated with once by daily oral gavage for one week. The biochemical indices and protein expression were measured. Herein, 79 constituents were identified in LJ, which were effective in elevating body weight, food consumption, water intake, and urinary excretion volume, as well as in ameliorating traumatic muscle tissues in model rats. In addition, LJ prominently decreased the contents of plasma viscosity, platelet aggregation rate, thrombin time, prothrombin time, activated partial thromboplastin time, fibrinogen, thromboxane B2 (TXB2), TXB2/6-keto-prostaglandin F1α (6-keto-PGF1α), urokinase-type plasminogen activator (u-PA), plasminogen activator inhibitor 1 (PAI-1), PAI-1/tissue-type PA (t-PA), and PAI-1/u-PA, while significantly increasing antithrombin III, 6-keto-PGF1α, and t-PA contents. Furthermore, LJ notably inhibited tumor necrosis factor alpha, interleukin 6 (IL-6), IL-8, angiotensin II, antidiuretic hormone, aldosterone, aquaporin 1 (AQP1), AQP2, and AQP3 levels, and markedly elevating IL-10 and natriuretic peptide levels. Finally, LJ markedly reduced the protein expression of AQP1, AQP2, and AQP3 compared to the model group. Collectively, LJ possessed prominent activating blood circulation, anti-inflammatory, and diuretic effects, thus supporting the clinical application of L. japonicus.
益母草传统上用于治疗多种疾病。本研究评估了益母草提取物（LJ）的活血化瘀、抗炎和利尿作用，并鉴定了其植物化学成分。在本研究中，采用液相色谱质谱联用技术鉴定了LJ中的植物化学成分。将大鼠随机分为三组（n = 8）：对照组给予生理盐水，模型组（生理盐水）和LJ组（426 mg/kg）诱导创伤性损伤。所有大鼠每天经口灌胃给药一次，持续一周。检测生化指标和蛋白表达。在此，在LJ中鉴定出79种成分，这些成分可有效提高体重、食物摄入量、饮水量和尿量，并改善模型大鼠的创伤性肌肉组织。此外，LJ显著降低血浆黏度、血小板聚集率、凝血酶时间、凝血酶原时间、活化部分凝血活酶时间、纤维蛋白原、血栓素B2（TXB2）、TXB2/6-酮-前列腺素F1α（6-酮-PGF1α）、尿激酶型纤溶酶原激活剂（u-PA）、纤溶酶原激活剂抑制剂1（PAI-1）、PAI-1/组织型PA（t-PA）和PAI-1/u-PA的含量，同时显著增加抗凝血酶III、6-酮-PGF1α和t-PA的含量。此外，LJ显著抑制肿瘤坏死因子α、白细胞介素6（IL-6）、IL-8、血管紧张素II、抗利尿激素、醛固酮、水通道蛋白1（AQP1）、AQP2和AQP3的水平，并显著提高IL-10和利钠肽水平。最后，与模型组相比，LJ显著降低AQP1、AQP2和AQP3的蛋白表达。总体而言，LJ具有显著的活血化瘀、抗炎和利尿作用，从而支持了益母草的临床应用。
