Antido Jhon Wilson A, Climacosa Fresthel Monica M
Department of Medical Microbiology, College of Public Health, University of the Philippines Manila, Manila 1000, Philippines.
Int J Microbiol. 2022 Oct 26;2022:2598963. doi: 10.1155/2022/2598963. eCollection 2022.
species are considered to be the most prolific sources of various bioactive secondary metabolites that are important for antibiotic production. Here, we describe a modified integrated approach to isolate species from diverse soil habitats, such as dumpsite, garden, forest, grassland, and riverside in Calamba City, Laguna, Philippines. A total of 25 soil samples were collected from a depth of 0-20 cm using systematic random soil sampling. All soil samples were air-dried, crushed, pretreated with calcium carbonate, and incubated on a rotary shaker. Isolation of in soil samples was then performed using the standard serial dilution plate technique on starch casein agar supplemented with nystatin (50 g/ml) and ampicillin (5 g/ml). Identification of the isolates was done using a polyphasic method that includes morphological and biochemical characterization. A total of 103 morphologically and biochemically distinct were isolated from diverse soil habitats. The number of isolates varied in each collection site, with the highest number collected from dumpsite soil and the least from forest soil. Most of the hydrogen sulfide producers were noted to be isolated from dumpsite samples. Moreover, more were isolated in soil habitats at higher altitudes with a slightly acidic to alkaline pH and a temperature ranging from 29 to 33°C. Employing the modified integrated approach, we have isolated up to 10 times more compared to early studies. These isolates can be valuable for future drug discovery and development research.
物种被认为是各种生物活性次生代谢物的最丰富来源，这些次生代谢物对抗生素生产很重要。在此，我们描述了一种改良的综合方法，用于从菲律宾拉古纳省卡兰巴市的各种土壤栖息地（如垃圾场、花园、森林、草地和河边）中分离物种。使用系统随机土壤采样方法，从0至20厘米深度共采集了25个土壤样本。所有土壤样本均进行风干、粉碎、用碳酸钙预处理，并在旋转摇床上孵育。然后使用标准系列稀释平板技术，在添加了制霉菌素（50微克/毫升）和氨苄青霉素（5微克/毫升）的淀粉酪蛋白琼脂上，对土壤样本中的物种进行分离。使用包括形态学和生化特征分析的多相方法对分离出的物种进行鉴定。从不同土壤栖息地共分离出103个形态和生化特征不同的物种。每个采集地点分离出的物种数量各不相同，其中从垃圾场土壤中采集到的数量最多，从森林土壤中采集到的数量最少。大多数硫化氢产生菌被发现是从垃圾场样本中分离出来的。此外，在海拔较高、pH值略呈酸性至碱性、温度范围为29至33°C的土壤栖息地中分离出了更多的物种。采用改良的综合方法，我们分离出的物种数量比早期研究多了多达10倍。这些分离出的物种对于未来的药物发现和开发研究可能具有重要价值。
