Raghava Rao Kothagorla Venkata, Raghava Rao Tamanam
Department of Biochemistry, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India.
J Young Pharm. 2013 Dec;5(4):121-6. doi: 10.1016/j.jyp.2013.10.002. Epub 2013 Dec 28.
To isolate and identify the biologically active strain of Streptomyces species from mangrove soil of Visakhapatnam region.
Actinomycetes are isolated by using starch casein agar media and four potential strains were selected to evaluate the antioxidant activity by using the standard methods DPPH, FRAP and total antioxidant capacity. Further, significant antioxidant activity strain characterized by morphological, physiological, biochemical and molecular characterization.
20 actinomycetes strains were isolated, among them four active isolates designated as BC 01, BC 02, BC 03 and BC 04 were studied for antioxidant activities. Of these four isolates, BC 01 showed a potent antioxidant activity when compared with other isolates. The morphological, biochemical and molecular characterization of the active isolate BC 01 belongs to the genus Streptomyces species. The phylogenetic tree was constructed and nucleotide blast in search indicated that the strain is 99.7% similarity with Streptomyces coelicoflavus.
The results of the present investigation proven that actinomycetes isolated from mangroves are potent source of antioxidants. The strain BC 01 exhibited a potential in vitro antioxidant activity; studies of actinomycetes from mangrove soil can be useful in discovery of novel species to get novel drugs.
从维沙卡帕特南地区的红树林土壤中分离并鉴定具有生物活性的链霉菌菌株。
使用淀粉酪蛋白琼脂培养基分离放线菌，并选择四个潜在菌株，采用DPPH、FRAP和总抗氧化能力等标准方法评估其抗氧化活性。此外，通过形态学、生理学、生物化学和分子特征对具有显著抗氧化活性的菌株进行表征。
分离出20株放线菌菌株，其中对四个活性分离株BC 01、BC 02、BC 03和BC 04进行了抗氧化活性研究。在这四个分离株中，与其他分离株相比，BC 01表现出较强的抗氧化活性。活性分离株BC 01的形态学、生物化学和分子特征表明其属于链霉菌属。构建了系统发育树，核苷酸比对搜索表明该菌株与天蓝色链霉菌的相似度为99.7%。
本研究结果证明，从红树林中分离出的放线菌是抗氧化剂的重要来源。菌株BC 01表现出潜在的体外抗氧化活性；对红树林土壤中的放线菌进行研究有助于发现新物种以获得新药物。
