Song Fuyang, Wu Yiming, Lin Xue, Xue Di, Wang Yujiong
Key Laboratory of The Ministry of Education for Conservation and Utilization of Special Biological Resources in The West, Ningxia University, Yinchuan, Ningxia Hui Autonomous Region 750021, P.R. China.
College of Life Science, Ningxia University, Yinchuan, Ningxia Hui Autonomous Region 750021, P.R. China.
Exp Ther Med. 2022 Oct 11;24(6):717. doi: 10.3892/etm.2022.11653. eCollection 2022 Dec.
Tuberculosis (TB) is a chronic and fatal zoonotic infectious disease caused by () infection. The THP-1 cell line is a cell model for studying the function, mechanism and signaling pathways of macrophages; macrophages are the primary host cells of . Macrophages are important for the progression of tuberculosis, as they affect the release of various inflammatory cytokines, including IL-1β, IL-6 and TNF-α. Vitamin C is a trace element for the human body. Its biological efficacy depends on its redox abilities and its role as a cofactor in several enzymatic reactions. However, whether vitamin C can protect THP-1 cells from infection has not yet been reported. The present study aimed to further investigate the effects of vitamin C on infection-induced THP-1 cell injury and its mechanism. In the present study, MTT assay, reverse transcription-quantitative PCR, EdU cell proliferation assay, western blotting, immunohistochemistry, flow cytometry and TUNEL staining assays were used to assess the cell viability, inflammation and apoptotic levels of THP-1 cells induced by following vitamin C treatment. The effect of vitamin C on infection was also assessed using Balb/c mice; pulmonary injury was assessed by H&E staining of the lung tissue. The results demonstrated that vitamin C markedly attenuated cellular damage caused by infection. The results demonstrated that vitamin C reduced the expression of -induced apoptosis-related proteins (Cleaved-caspase-9, Cleaved-caspase-3, Bcl-2, Cyt-c) and inflammatory factors (IL-1β, IL-6, NLRP3, TNF-α, IL-8, NF-κB) in THP-1 cells and reduced apoptosis. Overall, these results suggested that vitamin C may reduce lung damage caused by infection.
结核病（TB）是一种由（）感染引起的慢性致命性人畜共患传染病。THP-1细胞系是用于研究巨噬细胞功能、机制和信号通路的细胞模型；巨噬细胞是（）的主要宿主细胞。巨噬细胞对结核病的进展至关重要，因为它们会影响包括IL-1β、IL-6和TNF-α在内的多种炎性细胞因子的释放。维生素C是人体所需的微量元素。其生物学功效取决于其氧化还原能力及其在多种酶促反应中作为辅因子的作用。然而，维生素C是否能保护THP-1细胞免受（）感染尚未见报道。本研究旨在进一步探讨维生素C对（）感染诱导的THP-1细胞损伤的影响及其机制。在本研究中，采用MTT法、逆转录定量PCR、EdU细胞增殖检测、蛋白质免疫印迹法、免疫组织化学法、流式细胞术和TUNEL染色检测等方法，评估维生素C处理后（）诱导的THP-1细胞的活力、炎症和凋亡水平。还使用Balb/c小鼠评估了维生素C对（）感染的影响；通过对肺组织进行苏木精-伊红染色评估肺损伤情况。结果表明，维生素C显著减轻了（）感染引起的细胞损伤。结果表明，维生素C降低了（）诱导的THP-1细胞中凋亡相关蛋白（Cleaved-caspase-9、Cleaved-caspase-3、Bcl-2、Cyt-c）和炎性因子（IL-1β、IL-6、NLRP3、TNF-α、IL-8、NF-κB）的表达，并减少了细胞凋亡。总体而言，这些结果表明维生素C可能减轻（）感染引起的肺损伤。
