Wu Jiangling, Zhang Qiongyuan, Kang Lina, Wu Xiaotian, Li Daikun, Wang Yi, Huang Yu, Xue Jianjiang
Department of Clinical Laboratory, Medical Sciences Research Center, University-Town Hospital of Chongqing Medical University, Chongqing, 401331, China.
Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing 401331, China.
Anal Methods. 2022 Nov 24;14(45):4713-4720. doi: 10.1039/d2ay01523d.
Carcinoembryonic antigen (CEA) is regarded as a promising broad spectrum tumor biomarker for clinical diagnosis, progression, and prognosis. Surface plasmon resonance imaging (SPRi) was considered as one of the powerful tools for immunoassay with advantages of label-free, real-time detection with high-throughput. Herein, wavy gold-silver alloy nanoplates functionalized with anti-CEA antibodies providing high protein loading capacity and high mass are used as signal enhancers for CEA detection through SPRi sandwich assay. The present method exhibits a dynamic range for CEA determination from 0.1 to 312.5 ng mL and a detection limit of 0.55 ng mL, well below normal physiological levels. This biosensing approach demonstrates the advantages of wavy gold-silver alloy nanoplates compared to conventional gold nanoparticles as a signal amplifier to enhance the SPRi signal, which is expected to become a new prospect for detection of cancer markers in biomedical research and clinical diagnosis.
癌胚抗原（CEA）被视为一种用于临床诊断、病情进展及预后评估的有前景的广谱肿瘤生物标志物。表面等离子体共振成像（SPRi）被认为是免疫分析的强大工具之一，具有无需标记、高通量实时检测的优点。在此，用抗CEA抗体功能化的波浪形金银合金纳米片具有高蛋白负载能力和高质量，被用作通过SPRi夹心测定法检测CEA的信号增强剂。本方法对CEA测定的动态范围为0.1至312.5 ng/mL，检测限为0.55 ng/mL，远低于正常生理水平。这种生物传感方法展示了波浪形金银合金纳米片与传统金纳米颗粒相比作为信号放大器增强SPRi信号的优势，有望成为生物医学研究和临床诊断中癌症标志物检测的新前景。
