National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Japan Science and Technology Agency (JST), PRESTO, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan.
Chem Commun (Camb). 2022 Dec 1;58(96):13317-13320. doi: 10.1039/d2cc04919h.
The enzymatic luminescence reactions of fireflies are accelerated in the presence of biomolecular condensates comprising a positively charged peptide and ATP. We revealed that this acceleration is caused by the enrichment of reaction elements, local pH changes, and promotion of inhibitory intermediate dissociation, improving the bioluminescence quantum yield by approximately 10%.
萤火虫的酶促发光反应在包含带正电荷的肽和 ATP 的生物分子凝聚物的存在下会加速。我们揭示了这种加速是由反应元素的富集、局部 pH 值变化和促进抑制性中间物的解离引起的,从而将生物发光量子产率提高了约 10%。