Zhang Beibei, Zhao Wanxia, Tang Xiaoqin, Zhou Meng, Qiu Yanbo, Wang Shuhui, Sun Xiuzhu
College of Grassland Agriculture, Northwest A&F University, Yangling 712100, China.
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Vet Sci. 2025 Mar 18;12(3):283. doi: 10.3390/vetsci12030283.
This study aimed to identify insertion-deletion (InDel) variants in key genes of the Hippo signaling pathway in four Chinese sheep breeds: Tong sheep (TS), Hu sheep (HS), Small Tail Han sheep (STHS), and Lanzhou large-tailed sheep (LLTS). InDel variants in , , , , and genes were screened using public databases and identified through PCR amplification, gel electrophoresis, and sequencing. This study identified significant associations between InDel variants and growth traits across the four breeds. Specifically, three loci in the gene were significantly associated with chest circumference, body height, and body weight in STHS, HS, and TS. Four loci in influenced hip height, body weight, and chest circumference across all breeds ( < 0.05). Additionally, two loci in significantly affected body height, chest circumference, and body length in LLTS, STHS, and TS, while three loci in the gene strongly impacted cannon circumference in all breeds ( < 0.05). These InDel variants may serve as potential molecular markers for breeding. These findings highlight the potential of these InDel variants as molecular markers for sheep breeding and provide valuable resources for improving growth traits in sheep through molecular breeding.
本研究旨在鉴定4个中国绵羊品种，即同羊（TS）、湖羊（HS）、小尾寒羊（STHS）和兰州大尾羊（LLTS）中Hippo信号通路关键基因的插入缺失（InDel）变异。利用公共数据库筛选了 、 、 、 和 基因中的InDel变异，并通过聚合酶链反应（PCR）扩增、凝胶电泳和测序进行鉴定。本研究确定了InDel变异与这4个品种生长性状之间的显著关联。具体而言， 基因中的3个位点与STHS、HS和TS的胸围、体高和体重显著相关。 基因中的4个位点影响了所有品种的臀高、体重和胸围（ < 0.05）。此外， 基因中的2个位点显著影响了LLTS、STHS和TS的体高、胸围和体长，而 基因中的3个位点对所有品种的管围有强烈影响（ < 0.05）。这些InDel变异可能作为潜在的分子标记用于育种。这些发现突出了这些InDel变异作为绵羊育种分子标记的潜力，并为通过分子育种改善绵羊生长性状提供了有价值的资源。
