Zhao Guangsheng, Bi Mei, Liu Song, Ma Jian, Xu Fang, Liu Ying, Gao Fei, Yu Ying, Zhou Jun, Feng Zhuo, Wu Jianlin
Cancer Interventional Center, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.
Department of Clinical Nutrition and Metabolism, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.
Scand J Clin Lab Invest. 2022 Nov-Dec;82(7-8):549-555. doi: 10.1080/00365513.2022.2129438. Epub 2022 Nov 7.
Immune environment plays an important role in the management of liver cancer. The current study aimed to explore the change of NK and NKT cells, IL-17A, CD4 T and CD8 T cells in refractory liver metastases patients before and after CalliSpheres microspheres transarterial chemoembolization (CSM-TACE). Peripheral blood (PB) samples from 35 refractory liver metastases patients were collected before CSM-TACE (baseline), 2 days (D2) and 5 days (D5) after CSM-TACE. Then, NK and NKT cells, IL-17A, CD4 T and CD8 T cells from PB samples were detected. All enrolled patients successfully completed CSM-TACE procedure and achieved disease control rate of 100% after 1 month. NKT cells were increased from baseline to D2 and D5 [median (range): 5.88% (1.53%-12.05%) vs. 9.54% (5.19%-15.71%) vs. 7.12% (2.77%-13.29%)], NK cells were also enhanced from baseline to D2 and D5 [median (range): 14.35% (5.85%-20.52%) vs. 20.36% (15.88%-27.30%) vs. 30.82% (22.18%-37.72%)], while IL-17A was declined from baseline to D2 and D5 [median (range): 22.11 (9.46-39.18) pg/ml vs. 12.41 (3.24-26.84) pg/ml vs. 6.55 (1.11-20.98) pg/ml]. Furthermore, IL-17A was negatively correlated with the NK and NKT cells at baseline, D2 and D5 (all < .05), respectively. Additionally, CD4 T cells and CD4 T/CD8 T ratio were increased while CD8 T cells were declined from baseline to D2 and D5 (all < .05). NK cells, NKT cells, and CD4 T cells are increased but IL-17A and CD8 T cells are declined after CSM-TACE in refractory liver metastases.
免疫环境在肝癌治疗中发挥着重要作用。本研究旨在探讨载药微球经动脉化疗栓塞术（CSM-TACE）前后难治性肝转移患者自然杀伤细胞（NK）、自然杀伤T细胞（NKT）、白细胞介素-17A（IL-17A）、CD4⁺ T细胞和CD8⁺ T细胞的变化。收集35例难治性肝转移患者在CSM-TACE术前（基线）、术后2天（D2）和5天（D5）的外周血（PB）样本。然后，检测PB样本中的NK细胞、NKT细胞、IL-17A、CD4⁺ T细胞和CD8⁺ T细胞。所有入组患者均成功完成CSM-TACE手术，1个月后疾病控制率达100%。NKT细胞从基线到D2和D5增加[中位数（范围）：5.88%（1.53%-12.05%） vs. 9.54%（5.19%-15.71%） vs. 7.12%（2.77%-13.29%）]，NK细胞从基线到D2和D5也增强[中位数（范围）：14.35%（5.85%-20.52%） vs. 20.36%（15.88%-27.30%） vs. 30.82%（22.18%-37.72%）]，而IL-17A从基线到D2和D5下降[中位数（范围）：22.11（9.46-39.18）pg/ml vs. 12.41（3.24-26.84）pg/ml vs. 6.55（1.11-20.98）pg/ml]。此外，IL-17A在基线、D2和D5时分别与NK细胞和NKT细胞呈负相关（均P < 0.05）。另外，从基线到D2和D5，CD4⁺ T细胞和CD4⁺ T/CD8⁺ T细胞比值增加，而CD8⁺ T细胞减少（均P < 0.05）。难治性肝转移患者在CSM-TACE术后NK细胞、NKT细胞和CD4⁺ T细胞增加，但IL-17A和CD8⁺ T细胞减少。
