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在韧皮部顶端细胞中进行单细胞测序。

Rooting through single-cell sequencing in phloem pole cells.

机构信息

Department of Biology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.

出版信息

Commun Biol. 2022 Nov 7;5(1):1194. doi: 10.1038/s42003-022-04180-4.

DOI:10.1038/s42003-022-04180-4
PMID:36344596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9640702/
Abstract

While recent advances in plant single-cell RNA sequencing (scRNA-seq) have made numerous strides in identifying novel regulatory events, transcriptional profiling of certain cell types, such as phloem poles, has not yet been thoroughly investigated. A recent article by Otero et al. utilized cell-type specific marker lines and a second-generation single-cell approach to uncover transcriptomic landscapes specifying protophloem-adjacent cells, as well as identify a set of important transcription factors (TFs) signifying early phloem development.

摘要

虽然植物单细胞 RNA 测序 (scRNA-seq) 的最新进展在鉴定新的调控事件方面取得了许多进展,但某些细胞类型(如韧皮部极点)的转录谱尚未得到彻底研究。最近,Otero 等人利用细胞类型特异性标记线和第二代单细胞方法,揭示了指定原韧皮部相邻细胞的转录组图谱,并确定了一组重要的转录因子 (TFs),它们标志着早期韧皮部发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c185/9640702/22bbbe9dfda1/42003_2022_4180_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c185/9640702/22bbbe9dfda1/42003_2022_4180_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c185/9640702/22bbbe9dfda1/42003_2022_4180_Fig1_HTML.jpg

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本文引用的文献

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A root phloem pole cell atlas reveals common transcriptional states in protophloem-adjacent cells.根韧皮部柱细胞图谱揭示了与原韧皮部相邻细胞中的常见转录状态。
Nat Plants. 2022 Aug;8(8):954-970. doi: 10.1038/s41477-022-01178-y. Epub 2022 Aug 4.
2
Leaf cell-specific and single-cell transcriptional profiling reveals a role for the palisade layer in UV light protection.叶片细胞特异性和单细胞转录组分析揭示了栅栏层在紫外光保护中的作用。
Plant Cell. 2022 Aug 25;34(9):3261-3279. doi: 10.1093/plcell/koac167.
3
A single-cell Arabidopsis root atlas reveals developmental trajectories in wild-type and cell identity mutants.
一个拟南芥单细胞根系图谱揭示了野生型和细胞身份突变体的发育轨迹。
Dev Cell. 2022 Feb 28;57(4):543-560.e9. doi: 10.1016/j.devcel.2022.01.008. Epub 2022 Feb 7.