Kułak Karolina, Wojciechowska Natalia, Samelak-Czajka Anna, Jackowiak Paulina, Bagniewska-Zadworna Agnieszka
Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznanskiego 6, 61-614, Poznan, Poland.
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
Plant Methods. 2023 Nov 19;19(1):129. doi: 10.1186/s13007-023-01109-8.
The evolution of plants to efficiently transport water and assimilates over long distances is a major evolutionary success that facilitated their growth and colonization of land. Vascular tissues, namely xylem and phloem, are characterized by high specialization, cell heterogeneity, and diverse cell components. During differentiation and maturation, these tissues undergo an irreversible sequence of events, leading to complete protoplast degradation in xylem or partial degradation in phloem, enabling their undisturbed conductive function. Due to the unique nature of vascular tissue, and the poorly understood processes involved in xylem and phloem development, studying the molecular basis of tissue differentiation is challenging. In this review, we focus on methods crucial for gene expression research in conductive tissues, emphasizing the importance of initial anatomical analysis and appropriate material selection. We trace the expansion of molecular techniques in vascular gene expression studies and discuss the application of single-cell RNA sequencing, a high-throughput technique that has revolutionized transcriptomic analysis. We explore how single-cell RNA sequencing will enhance our knowledge of gene expression in conductive tissues.
植物进化出在长距离内有效运输水分和同化产物的能力是一项重大的进化成就，这促进了它们在陆地上的生长和定殖。维管组织，即木质部和韧皮部，具有高度特化、细胞异质性和多样的细胞成分的特点。在分化和成熟过程中，这些组织经历一系列不可逆转的事件，导致木质部原生质体完全降解或韧皮部部分降解，从而使其传导功能不受干扰。由于维管组织的独特性质以及木质部和韧皮部发育过程中涉及的过程了解甚少，研究组织分化的分子基础具有挑战性。在这篇综述中，我们重点关注传导组织中基因表达研究的关键方法，强调初始解剖分析和合适材料选择的重要性。我们追溯了分子技术在维管基因表达研究中的扩展，并讨论了单细胞RNA测序的应用，这是一种彻底改变转录组分析的高通量技术。我们探讨单细胞RNA测序将如何增强我们对传导组织中基因表达的认识。
