Burch Tanya C, Mackay Stephen, Hitefield Naomi L, Roberts Autumn B, Oduor Ian O, Nyalwidhe Julius O
Eastern Virginia Medical School.
Microbiology and Molecular Cell Biology.
MicroPubl Biol. 2022 Oct 22;2022. doi: 10.17912/micropub.biology.000658. eCollection 2022.
Prostate cancer clinical outcomes are varied, from non-aggressive asymptomatic to lethal aggressive neuroendocrine forms which represent a critical challenge in the management of the disease. The neurofilament light ( ) is proposed to be a tumor suppressor gene. Studies have shown that expression of the gene is decreased in various cancers. We have used quantitative RT-PCR, immunoblotting, methylation specific PCR, siRNA knockdown followed by migration/invasion assays to determine associations between expression and disease phenotype in a panel of prostate cells. We demonstrate that is overexpressed and it modulates invasion and migration in PC3-ML2 prostate cancers cells which have an aggressive neuroendocrine-like phenotype.
前列腺癌的临床结果各不相同，从非侵袭性无症状到致命的侵袭性神经内分泌形式，这对该疾病的管理构成了重大挑战。神经丝轻链（ ）被认为是一种肿瘤抑制基因。研究表明，该基因在各种癌症中的表达均降低。我们使用定量逆转录聚合酶链反应（qRT-PCR）、免疫印迹、甲基化特异性PCR、小干扰RNA（siRNA）敲低，随后进行迁移/侵袭试验，以确定一组前列腺细胞中 表达与疾病表型之间的关联。我们证明， 在具有侵袭性神经内分泌样表型的PC3-ML2前列腺癌细胞中过表达，并且它调节这些细胞的侵袭和迁移。
