College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China.
College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China.
PeerJ. 2022 Nov 2;10:e13962. doi: 10.7717/peerj.13962. eCollection 2022.
The impact of short-term nitrogen fertilizer input on the structure and diversity of peanut rhizosphere microbiota (RM) at different growth stages (GSs) was explored in the southern paddy soil planting environment. Three levels of nitrogen were applied in the field: control (LN, 0 kg/hm), medium nitrogen (MN, 55.68 kg/hm), and high nitrogen (HN, 111.36 kg/hm). The rhizosphere soil was collected during four GSs for high-throughput sequencing and chemical properties analysis. The effect of nitrogen fertilizer application on peanut RM was minimal and was obvious only at the seedling stage. In the four peanut GSs, a significant increase in relative abundance was observed for only one operational taxonomic unit (OTU) of under HN conditions at the seedling stage and mature stage, while there was no consistent change in other OTUs. The difference in RM among different peanut GSs was greater than that caused by the amount of nitrogen fertilizer. This may be due to the substantial differences in soil chemical properties (especially alkali-hydrolyzable nitrogen, pH, and available potassium or total potassium) among peanut GSs, as these significantly affected the RM structure. These results are of great value to facilitate deeper understanding of the effect of nitrogen fertilizer on peanut RM structure.
在南方稻田种植环境下，研究了短期氮肥投入对不同生长阶段（GS）花生根际微生物区系（RM）结构和多样性的影响。在田间施加了三个氮水平：对照（LN，0 kg/hm）、中氮（MN，55.68 kg/hm）和高氮（HN，111.36 kg/hm）。在四个 GS 期间采集根际土壤进行高通量测序和化学性质分析。氮肥对花生 RM 的影响很小，仅在苗期明显。在四个花生 GS 中，仅在 HN 条件下的幼苗期和成熟期观察到一个操作分类单元（OTU）的相对丰度显著增加，而其他 OTU 没有一致的变化。不同花生 GS 之间 RM 的差异大于氮肥用量引起的差异。这可能是由于花生 GS 之间土壤化学性质（特别是碱解氮、pH 值、有效钾或总钾）存在显著差异，这些差异显著影响了 RM 结构。这些结果对于深入了解氮肥对花生 RM 结构的影响具有重要价值。
