Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
ACS Sens. 2022 Nov 25;7(11):3243-3257. doi: 10.1021/acssensors.2c02165. Epub 2022 Nov 8.
Luminogens with aggregation-induced emission (AIEgens) properties have numerous broad applications in fields of chemical and biological analyses due to their exceptional photostability, excellent signal reliability, high quantum yield, and large Stokes' shift. In particular, AIEgens also bring new blood for immunoassay. Since publication of the first 2004 paper, AIEgens-based immunoassays have received significant attention because of their high sensitivity, specificity, accuracy, and reliability. However, until now, there have been no comprehensive literature reviews focused on the evolving field of AIEgens-based immunoassays. Thus, we have extensively reviewed AIEgens-based immunoassays from their basic working principles to specific applications. We focus on several fundamental elements of AIEgens-based immunoassays, including the typical structures of AIEgens, emission mechanism of AIEgens probes, function of AIEgens in immunoassays, and platform of AIEgens-based immunoassays. Then, the representative applications of AIEgens-based immunoassays in food safety, medical diagnostics, and environmental monitoring are explored. Thus, proposals on how to further improve the AIEgens-based immunoassay performance are also discussed, as well as future challenges and perspectives, aiming to provide brief and valid guidelines for choosing suitable AIEgens-based immunoassays according to specific application requirements.
具有聚集诱导发光（AIEgen）性质的发光体由于其非凡的光稳定性、优异的信号可靠性、高量子产率和大斯托克斯位移，在化学和生物分析领域有许多广泛的应用。特别是，AIEgen 也为免疫分析带来了新的血液。自 2004 年第一篇论文发表以来，基于 AIEgen 的免疫分析因其高灵敏度、特异性、准确性和可靠性而受到了极大的关注。然而，到目前为止，还没有全面的文献综述集中在基于 AIEgen 的免疫分析这一不断发展的领域。因此，我们从基本工作原理到具体应用广泛地综述了基于 AIEgen 的免疫分析。我们重点介绍了基于 AIEgen 的免疫分析的几个基本要素，包括 AIEgen 的典型结构、AIEgen 探针的发射机制、AIEgen 在免疫分析中的功能以及基于 AIEgen 的免疫分析平台。然后，探讨了基于 AIEgen 的免疫分析在食品安全、医学诊断和环境监测中的代表性应用。因此，还讨论了如何进一步提高基于 AIEgen 的免疫分析性能的建议，以及未来的挑战和展望，旨在根据特定的应用要求提供选择合适的基于 AIEgen 的免疫分析的简要有效的指南。
